Effect of repeated sciatic nerve crush on the conditioning lesion response: Generating an experimental animal model to prolong the denervation period while maintaining peripheral nerve continuity.

Autor: Minegishi Y; Physical Therapy Course, Department of Rehabilitation, Faculty of Health Sciences, Nihon Institute of Medical Science, Irumagun 350-0435, Japan; Graduate Course of Health and Social Services, Graduate School of Saitama Prefectural University, Koshigaya 343-8540, Japan., Ozone K; Graduate Course of Health and Social Services, Graduate School of Saitama Prefectural University, Koshigaya 343-8540, Japan; Department of Rehabilitation, University of Tsukuba Hospital, Tsukuba 305-8576, Japan., Oka Y; Department of Rehabilitation Science, Faculty of Health Sciences, Hokkaido University, Sapporo 060-0812, Japan., Kano T; Graduate Course of Health and Social Services, Graduate School of Saitama Prefectural University, Koshigaya 343-8540, Japan; Soka Orthopedic Internal Medicine, Soka 340-0016, Japan., Murata K; Department of Physical Therapy, Faculty of Health and Social Services, Saitama Prefectural University, Koshigaya 343-8540, Japan., Kanemura N; Department of Physical Therapy, Faculty of Health and Social Services, Saitama Prefectural University, Koshigaya 343-8540, Japan. Electronic address: kanemura-naohiko@spu.ac.jp.
Jazyk: angličtina
Zdroj: Neuroscience letters [Neurosci Lett] 2024 Jul 27; Vol. 836, pp. 137879. Date of Electronic Publication: 2024 Jun 14.
DOI: 10.1016/j.neulet.2024.137879
Abstrakt: Peripheral nerves exhibit long-term residual motor dysfunction following injury. The length of the denervation period before nerve and muscle reconnection is an important factor in motor function recovery. We aimed to investigate whether repeated nerve crush injuries to the same site every 7 days would preserve the conditioning lesion (CL) response and to determine the number of nerve crush injuries required to create an experimental animal model that would prolong the denervation period while maintaining peripheral nerve continuity. Rats were grouped according to the number of sciatic nerve crushes. A significant decrease in the soleus muscle fiber cross-sectional area was observed with increased crushes. After a single crush, macrophage accumulation and macrophage chemotaxis factor CCL2 expression in dorsal root ganglia were markedly increased, which aligned with the gene expression of Ccl2 and its receptor Ccr2. Macrophage numbers, histological CCL2 expression, and Ccl2 and Ccr2 gene expression levels decreased, depending on the number of repeated crushes. Histological analysis and gene expression analysis in the group with four repeated crushes did not differ significantly when compared with uninjured animals. Our findings indicated that repeated nerve crushes at the same site every 7 days sustained innervation loss and caused a loss of the CL response. The experimental model did not require nerve stump suturing and is useful for exploring factors causing prolonged denervation-induced motor dysfunction. SIGNIFICANCE STATEMENT: This study elucidates the effects of repeated nerve crush injury to the same site on innervation and conditioning lesion responses and demonstrates the utility of an experimental animal model that recapitulates the persistent residual motor deficits owing to prolonged denervation without requiring nerve transection and transection suturing.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: