α7 nicotinic receptor activation mitigates herpes simplex virus type 1 infection in microglia cells.

Autor: Chen SH; Viral Vector Core Facility, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA; Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA., Damborsky JC; Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA., Wilson BC; Viral Vector Core Facility, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA; Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA., Fannin RD; Molecular Genomics Core Facility, Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, Durham, NC, USA., Ward JM; Bioinformatics Support Group, Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, Durham, NC, USA., Gerrish KE; Molecular Genomics Core Facility, Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, Durham, NC, USA., He B; Viral Vector Core Facility, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA; Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA., Martin NP; Viral Vector Core Facility, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA; Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA., Yakel JL; Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA. Electronic address: yakel@niehs.nih.gov.
Jazyk: angličtina
Zdroj: Antiviral research [Antiviral Res] 2024 Aug; Vol. 228, pp. 105934. Date of Electronic Publication: 2024 Jun 15.
DOI: 10.1016/j.antiviral.2024.105934
Abstrakt: Herpes simplex virus type 1 (HSV-1), a neurotropic DNA virus, establishes latency in neural tissues, with reactivation causing severe consequences like encephalitis. Emerging evidence links HSV-1 infection to chronic neuroinflammation and neurodegenerative diseases. Microglia, the central nervous system's (CNS) immune sentinels, express diverse receptors, including α7 nicotinic acetylcholine receptors (α7 nAChRs), critical for immune regulation. Recent studies suggest α7 nAChR activation protects against viral infections. Here, we show that α7 nAChR agonists, choline and PNU-282987, significantly inhibit HSV-1 replication in microglial BV2 cells. Notably, this inhibition is independent of the traditional ionotropic nAChR signaling pathway. mRNA profiling revealed that choline stimulates the expression of antiviral factors, IL-1β and Nos2, and down-regulates the apoptosis genes and type A Lamins in BV2 cells. These findings suggest a novel mechanism by which microglial α7 nAChRs restrict viral infections by regulating innate immune responses.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Published by Elsevier B.V.)
Databáze: MEDLINE
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