Seasonal energy investment and metabolic patterns in a farmed fish.

Autor: Makri V; Laboratory of Animal Physiology, Department of Zoology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, Thessaloniki, GR-54124, Greece., Giantsis IA; Department of Animal Science, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, GR-54124, Thessaloniki, Greece., Nathanailides C; Department of Agriculture, University of Ioannina, GR-47100, Arta, Greece., Feidantsis K; Department of Fisheries & Aquaculture, University of Patras, GR-26504, Mesolonghi, Greece., Antonopoulou E; Laboratory of Animal Physiology, Department of Zoology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, Thessaloniki, GR-54124, Greece., Theodorou JA; Department of Fisheries & Aquaculture, University of Patras, GR-26504, Mesolonghi, Greece., Michaelidis B; Laboratory of Animal Physiology, Department of Zoology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, Thessaloniki, GR-54124, Greece. Electronic address: michaeli@bio.auth.gr.
Jazyk: angličtina
Zdroj: Journal of thermal biology [J Therm Biol] 2024 Jul; Vol. 123, pp. 103894. Date of Electronic Publication: 2024 Jun 13.
DOI: 10.1016/j.jtherbio.2024.103894
Abstrakt: The present research focuses on the seasonal changes in the energy content and metabolic patterns of red porgy (Pagrus pagrus) sampled in a fish farm in North Evoikos Gulf (Greece). The study was designed in an effort to evaluate the influence of seasonality in several physiological feauteres of high commercial importance that may affect feed intake and growth. We determined glycogen, lipids and proteins levels, and cellular energy allocation (CEA) as a valuable marker of exposure to stress, which integrates available energy (Ea) and energy consumption (Ec). Metabolic patterns and aerobic oxidation potential were based on the determination of glucose transporter (GLU), carnitine transporter (CTP), L-lactate dehydrogenase (L-LDH), citrate synthase (CS), cytochrome C oxidase subunit IV isoform 1 (COX1) and 3-hydroxyacyl CoA dehydrogenase (HOAD) relative gene expression. To integrate metabolic patterns and gene expression, L-LDH, CS, COX and HOAD activities were also determined. For further estimation of biological stores oxidized during seasonal acclimatization, we determined the blood levels of glucose, lipids and lactate. The results indicated seasonal changes in energy content, different patterns in gene expression and reorganization of metabolic patterns during cool acclimatization with increased lipid oxidation. During warm acclimatization, however, energy consumption was mostly based on carbohydrates oxidation. The decrease of E c and COX1 activity in the warm exposed heart seem to be consistent with the OCLTT hypothesis, suggesting that the heart may be one of the first organs to be limited during seasonal warming. Overall, this study has profiled changes in energetics and metabolic patterns occurring at annual temperatures at which P. pagrus is currently farmed, suggesting that this species is living at the upper edge of their thermal window, at least during summer.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Vasiliki Makri reports financial support was provided by Hellenic Foundation for Research and Innovation. John A. Theodorou reports financial support was provided by EU-Greece Operational Program of Fisheries. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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Databáze: MEDLINE
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