Naringin protects against paclitaxel-induced toxicity in rat testicular tissues by regulating genes in pro-inflammatory cytokines, oxidative stress, apoptosis, and JNK/MAPK signaling pathways.

Autor: Kankılıç NA; Department of Urology, Faculty of Medicine, Aksaray University, Aksaray, Turkey., Küçükler S; Department of Veterinary Biochemistry, Faculty of Veterinary, Atatürk University, Erzurum, Turkey., Gür C; Department of Medical Laboratory Techniques, Vocational School of Health Services, Atatürk University, Erzurum, Turkey., Akarsu SA; Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey., Akaras N; Department of Histology and Embryology, Faculty of Medicine, Aksaray University, Aksaray, Turkey., Şimşek H; Department of Physiology, Faculty of Medicine, Aksaray University, Aksaray, Turkey., İleritürk M; Department of Animal Science, Horasan Vocational College, Atatürk University, Erzurum, Turkey., Kandemir FM; Department of Medical Biochemistry, Faculty of Medicine, Aksaray University, Aksaray, Turkey.
Jazyk: angličtina
Zdroj: Journal of biochemical and molecular toxicology [J Biochem Mol Toxicol] 2024 Jul; Vol. 38 (7), pp. e23751.
DOI: 10.1002/jbt.23751
Abstrakt: Paclitaxel (PTX), which is actively used in the treatment of many types of cancer, has a toxic effect by causing increased oxidative stress in testicular tissues. Naringin (NRG) is a natural flavonoid found in plants, and its antioxidant properties are at the forefront. This study aims to investigate the protective feature of NRG in PTX-induced testicular toxicity. Thirty-five male Sprague rats were divided into five groups: control, NRG, PTX, PTX + NRG50, and PTX + NRG100. Rats were administered PTX (2 mg/kg, BW) intraperitoneally once daily for the first 5 days. Then, between the 6th and 14th days, NRG (50 and 100 mg/kg) was administered orally once a day. NRG reduced PTX-induced lipid peroxidation and increased testicular tissue antioxidant capacity (superoxide dismutase, catalase, glutathione peroxidase, and glutathione). While NRG reduces the mRNA expression levels of nuclear factor kappa B, tumor necrosis factor-alpha, interleukin-1 beta, cyclooxygenase-2, interleukin-6, inducible-nitric oxide synthase, mitogen-activated protein kinase 14 (MAPK)14, MAPK15, c-Jun N-terminal kinase, P53, Apaf1, Caspase3, Caspase6, Caspase9, and Bax in testicular tissues; it caused an increase in Nrf2, HO-1, NQO1 and Bcl-2 levels. NRG also improved the structural and functional integrity of testicular tissue disrupted by PTX. PTX-induced sperm damage was alleviated by NRG. NRG showed a protective effect by alleviating the PTX-induced testicular toxicity by increasing oxidative stress, inflammation, apoptosis, and autophagy.
(© 2024 The Author(s). Journal of Biochemical and Molecular Toxicology published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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