Dissecting the manipulation of lufenuron on chitin synthesis in Helicoverpa armigera.

Autor: Ma L; College of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang 330013, China., Zhao Z; College of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang 330013, China., Yang R; Institute of Entomology, Jiangxi Agricultural University, Nanchang 330045, China., Su Q; Institute of Entomology, Jiangxi Agricultural University, Nanchang 330045, China., Peng Y; Institute of Entomology, Jiangxi Agricultural University, Nanchang 330045, China., Zhang W; Institute of Entomology, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address: zhangwanna880210@yeah.net.
Jazyk: angličtina
Zdroj: Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2024 Jun; Vol. 202, pp. 105962. Date of Electronic Publication: 2024 May 16.
DOI: 10.1016/j.pestbp.2024.105962
Abstrakt: Lufenuron, a benzoylurea chitin synthesis inhibitor, is effective against many insect pests. However, the insecticidal activity of lufenuron has not been completely elucidated, nor has its disturbing effect on chitin synthesis genes. In this study, bioassay results demonstrated an outstanding toxicity of lufenuron against Helicoverpa armigera larvae. The treated larvae died from abortive molting and metamorphosis defects, and severe separation of epidermis and subcutaneous tissues was observed. Treatment of 3rd- and 4th-instar larvae with LC 25 lufenuron significantly extended the duration of larval and pupal stage, reduced the rates of pupation and emergence, and adversely affected pupal weight. Besides, lufenuron can severely reduce chitin content in larval integument, and the lufenuron-treated larvae showed reduced trehalose content in their hemolymph. Further analysis using RNA sequencing revealed that five chitin synthesis genes were down-regulated, whereas the expressions of two chitin degradation genes were significantly enhanced. Knockdown of chitin synthase 1 (HaCHS1), uridine diphosphate-N-acetylglucosamine-pyrophosphorylase (HaUAP), phosphoacetyl glucosamine mutase (HaPGM), and glucosamine 6-phosphate N-acetyl-transferase (HaGNPAT) in H. armigera led to significant increase in larval susceptibilities to LC 25 lufenuron by 75.48%, 65.00%, 68.42% and 28.00%, respectively. Our findings therefore revealed the adverse effects of sublethal doses of lufenuron on the development of H. armigera larvae, elucidated the perturbations on chitin metabolism, and proved that the combination of RNAi and lufenuron would improve the control effect of this pest.
Competing Interests: Declaration of competing interest The authors declare no competing interest.
(Copyright © 2023. Published by Elsevier Inc.)
Databáze: MEDLINE
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