SERS characterization of biochemical changes associated with biodesulfurization of dibenzothiophene using Gordonia sp. HS126-4N.

Autor: Umar Hussain M; Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan., Kainat K; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan., Nawaz H; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan. Electronic address: haqchemist@yahoo.com., Irfan Majeed M; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan. Electronic address: irfan.majeed@uaf.edu.pk., Akhtar N; Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan. Electronic address: nasrin@nibge.org., Alshammari A; Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Post Box 2455, Riyadh 11451, Saudi Arabia., Albekairi NA; Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Post Box 2455, Riyadh 11451, Saudi Arabia., Fatima R; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan., Amber A; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan., Bano A; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan., Shabbir I; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan., Tahira M; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan., Pallares RM; Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, Aachen 52074, Germany.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2024 Nov 05; Vol. 320, pp. 124534. Date of Electronic Publication: 2024 May 25.
DOI: 10.1016/j.saa.2024.124534
Abstrakt: In this study, Gordonia sp. HS126-4N was employed for dibenzothiophene (DBT) biodesulfurization, tracked over 9 days using SERS. During the initial lag phase, no significant spectral changes were observed, but after 48 h, elevated metabolic activity was evident. At 72 h, maximal bacterial population correlated with peak spectrum variance, followed by stable spectral patterns. Despite 2-hydroxybiphenyl (2-HBP) induced enzyme suppression, DBT biodesulfurization persisted. PCA and PLS-DA analysis of the SERS spectra revealed distinctive features linked to both bacteria and DBT, showcasing successful desulfurization and bacterial growth stimulation. PLS-DA achieved a specificity of 95.5 %, sensitivity of 94.3 %, and AUC of 74 %, indicating excellent classification of bacteria exposed to DBT. SERS effectively tracked DBT biodesulfurization and bacterial metabolic changes, offering insights into biodesulfurization mechanisms and bacterial development phases. This study highlights SERS' utility in biodesulfurization research, including its use in promising advancements in the field.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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