CDC48A, an interactor of WOX2, is required for embryonic patterning in Arabidopsis thaliana.

Autor: Gong W; Institute of Plant Sciences, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany.; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104, Freiburg, Germany., Bak DT; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104, Freiburg, Germany., Wendrich JR; Wageningen University, 6703, Wageningen, The Netherlands., Weijers D; Wageningen University, 6703, Wageningen, The Netherlands., Laux T; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104, Freiburg, Germany. laux@biologie.uni-freiburg.de.
Jazyk: angličtina
Zdroj: Plant cell reports [Plant Cell Rep] 2024 Jun 15; Vol. 43 (7), pp. 174. Date of Electronic Publication: 2024 Jun 15.
DOI: 10.1007/s00299-024-03158-2
Abstrakt: Key Message: Interactor of WOX2, CDC48A, is crucial for early embryo patterning and shoot meristem stem cell initiation, but is not required for WOX2 protein turnover or subcellular localization. During Arabidopsis embryo patterning, the WUSCHEL HOMEOBOX 2 (WOX2) transcription factor is a major regulator of protoderm and shoot stem cell initiation. Loss of WOX2 function results in aberrant protodermal cell divisions and, redundantly with its paralogs WOX1, WOX3, and WOX5, compromised shoot meristem formation. To elucidate the molecular basis for WOX2 function, we searched for protein interactors by IP-MS/MS from WOX2-overexpression roots displaying reprogramming toward shoot-like cell fates. Here, we report that WOX2 directly interacts with the type II AAA ATPase molecular chaperone CELL DIVISION CYCLE 48A (CDC48A). We confirmed this interaction with bimolecular fluorescence complementation and co-immunoprecipitation and found that both proteins co-localize in the nucleus. We show that CDC48A loss of function results in protoderm and shoot meristem stem cell initiation defects similar to WOX2 loss of function. We also provide evidence that CDC48A promotes WOX2 activity independently of proteolysis or the regulation of nuclear localization, common mechanisms of CDC48A function in other processes. Our results point to a new role of CDC48A in potentiating WOX2 function during early embryo patterning.
(© 2024. The Author(s).)
Databáze: MEDLINE
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