The endoplasmic reticulum connects to the nucleus by constricted junctions that mature after mitosis.
| Autor: | Bragulat-Teixidor H; Max Perutz Labs, Vienna Biocenter Campus (VBC), Vienna, Austria. helena.bragulatteixidor@meduniwien.ac.at.; Medical University of Vienna, Max Perutz Labs, Vienna, Austria. helena.bragulatteixidor@meduniwien.ac.at.; Vienna BioCenter PhD Program, a Doctoral School of the University of Vienna and Medical University of Vienna, Vienna, Austria. helena.bragulatteixidor@meduniwien.ac.at., Ishihara K; Department of Computational and Systems Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA., Szücs GM; Max Perutz Labs, Vienna Biocenter Campus (VBC), Vienna, Austria.; Medical University of Vienna, Max Perutz Labs, Vienna, Austria., Otsuka S; Max Perutz Labs, Vienna Biocenter Campus (VBC), Vienna, Austria. shotaro.otsuka@univie.ac.at.; Medical University of Vienna, Max Perutz Labs, Vienna, Austria. shotaro.otsuka@univie.ac.at. |
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| Jazyk: | angličtina |
| Zdroj: | EMBO reports [EMBO Rep] 2024 Jul; Vol. 25 (7), pp. 3137-3159. Date of Electronic Publication: 2024 Jun 14. |
| DOI: | 10.1038/s44319-024-00175-w |
| Abstrakt: | Junctions between the endoplasmic reticulum (ER) and the outer membrane of the nuclear envelope (NE) physically connect both organelles. These ER-NE junctions are essential for supplying the NE with lipids and proteins synthesized in the ER. However, little is known about the structure of these ER-NE junctions. Here, we systematically study the ultrastructure of ER-NE junctions in cryo-fixed mammalian cells staged in anaphase, telophase, and interphase by correlating live cell imaging with three-dimensional electron microscopy. Our results show that ER-NE junctions in interphase cells have a pronounced hourglass shape with a constricted neck of 7-20 nm width. This morphology is significantly distinct from that of junctions within the ER network, and their morphology emerges as early as telophase. The highly constricted ER-NE junctions are seen in several mammalian cell types, but not in budding yeast. We speculate that the unique and highly constricted ER-NE junctions are regulated via novel mechanisms that contribute to ER-to-NE lipid and protein traffic in higher eukaryotes. (© 2024. The Author(s).) |
| Databáze: | MEDLINE |
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