An IgG1 single-dilution avidity ELISA predicts cross-protection against heterologous foot-and-mouth disease virus challenge after vaccination.

Autor: Cardoso N; Institute of Virology and Technical Innovations, INTA-CONICET, Nicolás Repetto y Los Reseros S/N Hurlingham, 1686 Buenos Aires, Argentina., Eschbaumer M; Institute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, Suedufer 10, 17493 Greifswald-Insel Riems, Germany., Capozzo AV; Institute of Virology and Technical Innovations, INTA-CONICET, Nicolás Repetto y Los Reseros S/N Hurlingham, 1686 Buenos Aires, Argentina. Electronic address: alejandra_capozzo@yahoo.com.ar.
Jazyk: angličtina
Zdroj: Vaccine [Vaccine] 2024 Nov 14; Vol. 42 (25), pp. 126066. Date of Electronic Publication: 2024 Jun 14.
DOI: 10.1016/j.vaccine.2024.06.033
Abstrakt: This study aims to analyze if the results from different serological assays, used alone or combined, could match the outcome of challenge infection with foot-and-mouth disease virus (FMDV) after vaccination in cattle. Day-of-challenge sera from animals that had been vaccinated 21 days before with monovalent formulations containing inactivated A Iran 96 or A Iran 99 virus strains were used. Challenge and serology were performed with A22 Iraq strain. IgG1 titers and total-IgG avidity indexes were significantly higher in protected animals (p < 0.01) while IgG2-titers were not related to protection (p > 0.05). An IgG1 avidity ELISA was developed to analyze in one step, IgG1 levels and avidity. This assay estimated protection with 96 % accuracy. A strong agreement with challenge results was achieved (K = 0.85), suggesting a role of high-affinity IgG1 in protection against FMDV. These results support the assessment of the single dilution IgG1-Avidity ELISA to predict cross-protection in FMDV-vaccinated cattle.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Nancy Cardoso reports financial support and travel were provided by Global Foot and Mouth Disease Research Alliance. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: