Ultrasensitive and multiplexed tracking of single cells using whole-body PET/CT.

Autor:	Nguyen HTM; School of Medicine, Department of Radiation Oncology and Medical Physics, Stanford University, Stanford, CA 94305, USA., Das N; School of Medicine, Department of Radiation Oncology and Medical Physics, Stanford University, Stanford, CA 94305, USA., Ricks M; School of Medicine, Department of Radiological Sciences, Stanford University, Stanford, CA 94305, USA., Zhong X; School of Medicine, Department of Radiation Oncology and Medical Physics, Stanford University, Stanford, CA 94305, USA., Takematsu E; School of Medicine, Department of Surgery, Stanford University, Stanford, CA 94305, USA., Wang Y; School of Medicine, Department of Surgery, Stanford University, Stanford, CA 94305, USA., Ruvalcaba C; Department of Biomedical Engineering, University of California, Davis, Davis, CA 95616, USA., Mehadji B; Department of Radiology, University of California, Davis, Davis, CA 95616, USA., Roncali E; Department of Biomedical Engineering, University of California, Davis, Davis, CA 95616, USA.; Department of Radiology, University of California, Davis, Davis, CA 95616, USA., Chan CKF; School of Medicine, Department of Surgery, Stanford University, Stanford, CA 94305, USA., Pratx G; School of Medicine, Department of Radiation Oncology and Medical Physics, Stanford University, Stanford, CA 94305, USA.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2024 Jun 14; Vol. 10 (24), pp. eadk5747. Date of Electronic Publication: 2024 Jun 14.
DOI:	10.1126/sciadv.adk5747
Abstrakt:	In vivo molecular imaging tools are crucially important for elucidating how cells move through complex biological systems; however, achieving single-cell sensitivity over the entire body remains challenging. Here, we report a highly sensitive and multiplexed approach for tracking upward of 20 single cells simultaneously in the same subject using positron emission tomography (PET). The method relies on a statistical tracking algorithm (PEPT-EM) to achieve a sensitivity of 4 becquerel per cell and a streamlined workflow to reliably label single cells with over 50 becquerel per cell of 18 F-fluorodeoxyglucose (FDG). To demonstrate the potential of the method, we tracked the fate of more than 70 melanoma cells after intracardiac injection and found they primarily arrested in the small capillaries of the pulmonary, musculoskeletal, and digestive organ systems. This study bolsters the evolving potential of PET in offering unmatched insights into the earliest phases of cell trafficking in physiological and pathological processes and in cell-based therapies.
Databáze:	MEDLINE
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