Possible role of intestinal fungal dysbiosis in dectin-1 and cytokines expression in patients with ulcerative colitis.

Autor: Azizollah N; Student Research Committee, Alborz University of Medical Sciences, Karaj, Iran., Sharifinejad N; Student Research Committee, Alborz University of Medical Sciences, Karaj, Iran.; Non-Communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, Iran., Mozhgani SH; Non-Communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, Iran.; Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran., Mousavian SM; Department of Gastroenterology and Hepatology, Tehran University of Medical Sciences, Tehran, Iran., Bakhtiyari M; Non-Communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, Iran.; Department of Community Medicine, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran., Mahmoudi E; Department of Mycology, School of Medicine, Alborz University of Medical Sciences, Taleghani Boulevard, Taleghani Square, Karaj, Iran. e_m592000@yahoo.com.
Jazyk: angličtina
Zdroj: Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology [Indian J Gastroenterol] 2024 Aug; Vol. 43 (4), pp. 832-840. Date of Electronic Publication: 2024 Jun 14.
DOI: 10.1007/s12664-024-01605-2
Abstrakt: Background: Dysregulation of cytokines and intestinal mycobiome has been surveyed in the progression of inflammatory bowel diseases (IBDs), including ulcerative colitis (UC) and Crohn's disease (CD). On the other hand, the intestinal fungal flora and its main receptor, Dectin-1, induce immune-derived cytokines.
Methods: Total 64 individuals comprising 32 patients with UC (case group) and 32 healthy subjects (HS group) were assessed. The type and prevalence of fecal yeast species were determined by deoxyribonucleic acid (DNA) sequencing through polymerase chain reaction (PCR) amplification using ITS4 and ITS5 primers. Furthermore, the ribonucleic acid (RNAs) of IL-4, IL-10, IL-17, IL-22 and IFN-γ were extracted. The expression of Dectin-1 gene was then measured in the excised tissue samples.
Results: A higher global fungal load in UC-affected patients (75%) was found in comparison with the HS group (25%), especially Candida albicans. Saccharomyces cerevisiae was significantly reduced in the fecal samples of UC-affected patients compared to HS (15.04% vs. 1.93% UC). The expression level of Dectin-1 was significantly elevated in patients with active UC (7.37 ± 0.81) than in patients with non-active UC (5.01 ± 77.25) and healthy controls (0.97 ± 0.24) (p < 0.05). The expression levels of IL-4, IL-10, especially both IL-17 and IL-22, were higher in the active UC group compared to the HS group (p = 0.0101, p = 0.0155, p < 0.0001, p < 0.0001, respectively). Similar expression level of IL-4, IL-10, IL-17, IL-22 (p > 0.999) and lower expression of interferongamma (IFN-γ) (p = 0.0021) were found in the non-active UC group compared to the HS group. A significant weak to moderate correlation was detected between Dectin-1 and IL-17 (r = 0.339, p = 0.019), as well as Dectin-1 and IL-22 (r = 0.373, p = 0.015). Furthermore, the expression levels of Dectin-1, IL-17 and IL-22 displayed significant associations with disease activity (p < 0.001, p = 0.029 and p = 0.003, respectively), regardless of the participant group.
Conclusions: The current study revealed a possible role for intestinal fungi to promote colonic inflammation and increase UC activity through Dectin-1 stimulation. A positive correlation was detected between intestinal fungal richness with UC susceptibility and activity. IL-4 and IL-10 were associated with disease activity. Besides, the expression levels of Dectin-1, IL-17 and IL-22 were independently associated with disease activity.
(© 2024. Indian Society of Gastroenterology.)
Databáze: MEDLINE
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