Fortified electrospun collagen utilizing biocompatible Poly Glycerol Sebacate prepolymer (PGSp) and zink oxide nanoparticles (ZnO NPs) for diabetics wound healing: Physical, biological and animal studies.

Autor:	Larijani G; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran., Parivar K; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran., Hayati Roodbari N; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran., Yaghmaei P; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran., Amini N; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Jazyk:	angličtina
Zdroj:	Regenerative therapy [Regen Ther] 2024 May 29; Vol. 26, pp. 102-113. Date of Electronic Publication: 2024 May 29 (Print Publication: 2024).
DOI:	10.1016/j.reth.2024.05.009
Abstrakt:	Collagen, a naturally occurring fibrous protein, is a potential resource of biological materials for tissue engineering and regenerative medicine because it is structurally biocompatible, has low immunogenicity, is biodegradable, and is biomimetic. Numerous studies have documented in the literature how Collagen nanofibers exhibit limited cell adhesion, poor viscosity, and no interior fibril structure. The biomedical industry is using Poly Glycerol Sebacate prepolymer(PGSp), a biodegradable and biocompatible polyester with high adhesion and very viscous appearance, more often. Here, unique electrospun Collagen/PGSp/ZnO/NPs blend nanofibers for skin tissue application were developed and described with varied PGSp percent. Additionally, when ternary blends of Collagen, PGSp, and Zink Oxide Nanoparticles (ZnO NPs) are used, the antibacterial properties of the scaffolds are improved. The bead-free electrospun nanofibers were produced by raising the PGSp concentration to 30%w/w. SEM, EDS, tensile, MTT, FTIR, SDS-page, swelling test, contact-angle, antimicrobial, biodegradation, XRD, and cell attachment procedures were used to characterize the crosslinked nanofibers. The ternary blend nanofibers with a weight ratio of Collagen/PGSp 30%/ZnONPs 1% had higher stress/strain strength (0.25 mm/mm), porosity (563), cell survival, and degradation time. Moreover, after applying for wound healing in diabetic rats, Collagen/PGSp 30%/could be show improving wound healing significantly compared to other groups. Competing Interests: No conflict of interest exits in the submission of this manuscript, and manuscript is approved by all authors for publication. (© 2024 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.)
Databáze:	MEDLINE
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