Serum cytokine dysregulation signatures associated with COVID-19 outcomes in high mortality intensive care unit cohorts across pandemic waves and variants.

Autor: Maaß H; Department of Viral Immunology, Helmholtz Center for Infection Research, Braunschweig, Germany.; Centre for Individualized Infection Medicine (CiiM), a joint venture of Helmholtz Centre for Infection Research and Hannover Medical School, Hannover, Germany., Ynga-Durand M; Department of Viral Immunology, Helmholtz Center for Infection Research, Braunschweig, Germany.; Centre for Individualized Infection Medicine (CiiM), a joint venture of Helmholtz Centre for Infection Research and Hannover Medical School, Hannover, Germany., Milošević M; Department of Anesthesiology, Faculty of Medicine, Reanimation, Intensive Care and Emergency Medicine, University of Rijeka, Rijeka, Croatia., Krstanović F; Faculty of Medicine, Center for Proteomics, University of Rijeka, Rijeka, Croatia., Matešić MP; Faculty of Medicine, Center for Proteomics, University of Rijeka, Rijeka, Croatia., Žuža I; Department of Radiology, Clinical Hospital Centre Rijeka, Rijeka, Croatia., Jonjić S; Faculty of Medicine, Center for Proteomics, University of Rijeka, Rijeka, Croatia., Brizić I; Faculty of Medicine, Center for Proteomics, University of Rijeka, Rijeka, Croatia., Šustić A; Department of Anesthesiology, Faculty of Medicine, Reanimation, Intensive Care and Emergency Medicine, University of Rijeka, Rijeka, Croatia.; Department of Clinical Medical Science II, Faculty of Health Studies, University of Rijeka, Rijeka, Croatia., Bloos F; Department of Anesthesiology and Intensive Care Medicine, Jena University Hospital, Jena, Germany., Protić A; Department of Anesthesiology, Faculty of Medicine, Reanimation, Intensive Care and Emergency Medicine, University of Rijeka, Rijeka, Croatia., Čičin-Šain L; Department of Viral Immunology, Helmholtz Center for Infection Research, Braunschweig, Germany. Luka.Cicin-Sain@helmholtz-hzi.de.; Centre for Individualized Infection Medicine (CiiM), a joint venture of Helmholtz Centre for Infection Research and Hannover Medical School, Hannover, Germany. Luka.Cicin-Sain@helmholtz-hzi.de.; German Centre for Infection Research (DZIF), Partner Site Hannover/Braunschweig, Braunschweig, Germany. Luka.Cicin-Sain@helmholtz-hzi.de.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jun 13; Vol. 14 (1), pp. 13605. Date of Electronic Publication: 2024 Jun 13.
DOI: 10.1038/s41598-024-64384-y
Abstrakt: The aim of this study was to characterize the systemic cytokine signature of critically ill COVID-19 patients in a high mortality setting aiming to identify biomarkers of severity, and to explore their associations with viral loads and clinical characteristics. We studied two COVID-19 critically ill patient cohorts from a referral centre located in Central Europe. The cohorts were recruited during the pre-alpha/alpha (November 2020 to April 2021) and delta (end of 2021) period respectively. We determined both the serum and bronchoalveolar SARS-CoV-2 viral load and identified the variant of concern (VoC) involved. Using a cytokine multiplex assay, we quantified systemic cytokine concentrations and analyzed their relationship with clinical findings, routine laboratory workup and pulmonary function data obtained during the ICU stay. Patients who did not survive had a significantly higher systemic and pulmonary viral load. Patients infected with the pre-alpha VoC showed a significantly lower viral load in comparison to those infected with the alpha- and delta-variants. Levels of systemic CTACK, M-CSF and IL-18 were significantly higher in non-survivors in comparison to survivors. CTACK correlated directly with APACHE II scores. We observed differences in lung compliance and the association between cytokine levels and pulmonary function, dependent on the VoC identified. An intra-cytokine analysis revealed a loss of correlation in the non-survival group in comparison to survivors in both cohorts. Critically ill COVID-19 patients exhibited a distinct systemic cytokine profile based on their survival outcomes. CTACK, M-CSF and IL-18 were identified as mortality-associated analytes independently of the VoC involved. The Intra-cytokine correlation analysis suggested the potential role of a dysregulated systemic network of inflammatory mediators in severe COVID-19 mortality.
(© 2024. The Author(s).)
Databáze: MEDLINE
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