Disruption of the cell division protein ftsK gene changes elemental selenium generation, selenite tolerance, and cell morphology in Rahnella aquatilis HX2.

Autor:	Xu Q; College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.; Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China., Gao S; College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.; Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China., Zhang S; College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.; Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China., Li K; College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.; Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China., Guo Y; College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.; Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China.
Jazyk:	angličtina
Zdroj:	Journal of applied microbiology [J Appl Microbiol] 2024 Jun 03; Vol. 135 (6).
DOI:	10.1093/jambio/lxae142
Abstrakt:	Aims: Some studies have indicated that the alterations in cellular morphology induced by selenite [Se(Ⅳ)] may be attributed to its inhibitory effects on cell division. However, whether the genes associated with cell division are implicated in Se(Ⅳ) metabolism remains unclear. Methods and Results: The ftsK gene in Rahnella aquatilis HX2 was mutated with an in-frame deletion strategy. The ftsK mutation strongly reduced the tolerance to selenite [Se(Ⅳ)] and the production of red elemental selenium [Se(0)] in R. aquatilis HX2, and this effect could not be attributed solely to the inhibition of cell growth. Deleting the ftsK gene also resulted in a significant decrease in bacterial growth of R. aquatilis HX2 during both exponential and stationary phases. The deletion of ftsK inhibited cell division, resulting in the development of elongated filamentous cells. Furthermore, the loss-of-function of FtsK significantly impacted the expression of seven genes linked to cell division and Se(Ⅳ) metabolism by at least 2-fold, as unveiled by real-time quantitative PCR (RT-qPCR) under Se(Ⅳ) treatment. Conclusions: These findings suggest that FtsK is associated with Se(Ⅳ) tolerance and Se(0) generation and is a key player in coordinating bacterial growth and cell morphology in R. aquatilis HX2. (© The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu