Exploring bioluminescence in Aglaonema: Investigating Vibrio campbellii translocation and plant responses under CaCl₂ stimulation.

Autor: Dolphen R; Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand. Electronic address: rujira.dol@kmutt.ac.th., Treesubsuntorn C; School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand., Kanjanapokin C; Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand., Chonjoho N; School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand., Anusaraporn S; Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand., Julpanwattana P; Research and Innovation for Sustainability Center (RISC), Magnolia Quality Development Corporation Limited (MQDC), Thailand., Praditsmanont A; Research and Innovation for Sustainability Center (RISC), Magnolia Quality Development Corporation Limited (MQDC), Thailand.
Jazyk: angličtina
Zdroj: Environmental research [Environ Res] 2024 Sep 15; Vol. 257, pp. 119414. Date of Electronic Publication: 2024 Jun 12.
DOI: 10.1016/j.envres.2024.119414
Abstrakt: The feasibility of creating light-emitting plants by immobilizing Vibrio campbellii RMT1 on the rhizospheric zone of Aglaonema sp. 'Banlangngoen' was investigated in depth, including bacteria translocation and plant response. Results from scanning electron microscope showed that an inorganic salt-containing medium affected the root. However, transmission electron microscope results displayed bacteria translocation through the root to the leaf and colonized in the cytosol of vascular tissues. Bacteria cell counts exhibited high colonization in the root zone, approximately 3.65 × 10 6  CFU/mL, resulting in a light-emitting intensity increase of 23.68-fold higher than the control after the first week. Nevertheless, light microscope revealed that inorganic salts in the culture medium led to enlarged air spaces, resulting in leaf and stalk withering. Notably, spraying plants with calcium chloride (CaCl 2 ) solution effectively mitigated salt stress, activated luminescence, and facilitated bacterial movement from roots to leaves. Additionally, CaCl 2 contributed to ongoing salinity reduction in the culture medium, as evidenced by reduced malondialdehyde levels, alongside increased indole-3-acetic acid and salicylic acid concentrations, indicating plant defense responses. The interaction between plants and luminescent bacteria demonstrated the potential for producing glowing plants following CaCl 2 application, addressing salinity stress, enhancing luminescence, and maintaining plant growth.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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