Tropoelastin modulates systemic and local tissue responses to enhance wound healing.

Autor: Wang Z; School of Life and Environmental Sciences, the University of Sydney, NSW 2006, Australia; Charles Perkins Centre, the University of Sydney, NSW 2006, Australia., Shi H; Burns Research and Reconstructive Surgery, Anzac Research Institute, NSW 2139, Australia; Asbestos and Dust Disease Research Institute, Concord Hospital, Sydney, NSW 2139, Australia., Silveira PA; Dendritic Cell Group, ANZAC Research Institute, Concord Hospital, Sydney, NSW 2139, Australia., Mithieux SM; School of Life and Environmental Sciences, the University of Sydney, NSW 2006, Australia; Charles Perkins Centre, the University of Sydney, NSW 2006, Australia., Wong WC; Charles Perkins Centre, the University of Sydney, NSW 2006, Australia., Liu L; School of Life and Environmental Sciences, the University of Sydney, NSW 2006, Australia; Charles Perkins Centre, the University of Sydney, NSW 2006, Australia., Pham NTH; Key Centre for Polymers and Colloids, School of Chemistry, the University of Sydney, NSW 2006, Australia., Hawkett BS; Key Centre for Polymers and Colloids, School of Chemistry, the University of Sydney, NSW 2006, Australia., Wang Y; Burns Research and Reconstructive Surgery, Anzac Research Institute, NSW 2139, Australia; Jiangsu Provincial Engineering Research Centre of TCM External Medication Development and Application, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China. Electronic address: yiweiwang@njucm.edu.cn., Weiss AS; School of Life and Environmental Sciences, the University of Sydney, NSW 2006, Australia; Charles Perkins Centre, the University of Sydney, NSW 2006, Australia; The University of Sydney Nano Institute, the University of Sydney, NSW 2006, Australia. Electronic address: tony.weiss@sydney.edu.au.
Jazyk: angličtina
Zdroj: Acta biomaterialia [Acta Biomater] 2024 Aug; Vol. 184, pp. 54-67. Date of Electronic Publication: 2024 Jun 11.
DOI: 10.1016/j.actbio.2024.06.009
Abstrakt: Wound healing is facilitated by biomaterials-based grafts and substantially impacted by orchestrated inflammatory responses that are essential to the normal repair process. Tropoelastin (TE) based materials are known to shorten the period for wound repair but the mechanism of anti-inflammatory performance is not known. To explore this, we compared the performance of the gold standard Integra Dermal Regeneration Template (Integra), polyglycerol sebacate (PGS), and TE blended with PGS, in a murine full-thickness cutaneous wound healing study. Systemically, blending with TE favorably increased the F4/80 + macrophage population by day 7 in the spleen and contemporaneously induced elevated plasma levels of anti-inflammatory IL-10. In contrast, the PGS graft without TE prompted prolonged inflammation, as evidenced by splenomegaly and greater splenic granulocyte and monocyte fractions at day 14. Locally, the inclusion of TE in the graft led to increased anti-inflammatory M2 macrophages and CD4 + T cells at the wound site, and a rise in Foxp3 + regulatory T cells in the wound bed by day 7. We conclude that the TE-incorporated skin graft delivers a pro-healing environment by modulating systemic and local tissue responses. STATEMENT OF SIGNIFICANCE: Tropoelastin (TE) has shown significant benefits in promoting the repair and regeneration of damaged human tissues. In this study, we show that TE promotes an anti-inflammatory environment that facilitates cutaneous wound healing. In a mouse model, we find that inserting a TE-containing material into a full-thickness wound results in defined, pro-healing local and systemic tissue responses. These findings advance our understanding of TE's restorative value in tissue engineering and regenerative medicine, and pave the way for clinical applications.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: A.S.W. is the founding scientist of Elastagen Pty. Ltd., now sold to Allergan, Inc., an AbbVie company.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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