Assessment of NIR-triggered PEG-coated NaGdF 4 :Tm 3+ /Yb 3+ bio-compatible upconversion nanoparticles for contrast enhancement in OCT imaging and optical thermometry.

Autor: Shwetabh K; Optical Materials and Bio-imaging Research Laboratory, Department of Physics, Indian Institute of Technology (Indian School of Mines), Dhanbad 826004, India., Banerjee A; Biophotonics Lab, Department of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand 835215, India., Poddar R; Biophotonics Lab, Department of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand 835215, India., Kumar K; Optical Materials and Bio-imaging Research Laboratory, Department of Physics, Indian Institute of Technology (Indian School of Mines), Dhanbad 826004, India.
Jazyk: angličtina
Zdroj: Biomedical materials (Bristol, England) [Biomed Mater] 2024 Jul 03; Vol. 19 (5). Date of Electronic Publication: 2024 Jul 03.
DOI: 10.1088/1748-605X/ad580b
Abstrakt: In this investigation, we embarked on the synthesis of polyethylene glycol coated NaGdF 4 :Tm 3+ /Yb 3+ upconversion nanoparticles (UCNPs), aiming to assess their utility in enhancing image contrast within the context of swept source optical coherence tomography (OCT) and photo-thermal OCT imaging. Our research unveiled the remarkable UC emissions stemming from the transitions of Tm 3+ ions, specifically the 1 G 4 → 3 H 6 transitions, yielding vibrant blue emissions at 472 nm. We delved further into the UC mechanism, meticulously scrutinizing decay times and the nanoparticles' capacity to convert radiation into heat. Notably, these nanoparticles exhibited an impressive photo-thermal conversion efficiency of 37.5%. Furthermore, our investigations into their bio-compatibility revealed a promising outcome, with more than 90% cell survival after 24 h of incubation with HeLa cells treated with UCNPs. The nanoparticles demonstrated a notable thermal sensitivity of 4.7 × 10 -3 K -1 at 300 K, signifying their potential for precise temperature monitoring at the cellular level.
(© 2024 IOP Publishing Ltd.)
Databáze: MEDLINE
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