Potential usage of boron modified carbon nanodots as a marker candidate for coronavirus disease (COVID-19) antibody detection.

Autor: Wibrianto A; Department of Chemistry, Universitas Airlangga, Surabaya 60115, Indonesia; Supra Modification Nano-Micro Engineering Research Group, Universitas Airlangga, Surabaya 60115, Indonesia., Saputra YJ; Department of Chemistry, Universitas Airlangga, Surabaya 60115, Indonesia., Sugito SFA; Department of Chemistry, Universitas Airlangga, Surabaya 60115, Indonesia; Supra Modification Nano-Micro Engineering Research Group, Universitas Airlangga, Surabaya 60115, Indonesia., Khairunisa SQ; Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia., Rachman BE; Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia; Faculty of Medicine, Universitas Airlangga, Surabaya 60132, Indonesia., Nasronudin N; Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia; Faculty of Medicine, Universitas Airlangga, Surabaya 60132, Indonesia., Megasari NLA; Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia., Chang JY; Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106335, Taiwan, Republic of China., Fahmi MZ; Department of Chemistry, Universitas Airlangga, Surabaya 60115, Indonesia; Supra Modification Nano-Micro Engineering Research Group, Universitas Airlangga, Surabaya 60115, Indonesia. Electronic address: m.zakki.fahmi@fst.unair.ac.id.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2024 Sep 15; Vol. 248, pp. 116242. Date of Electronic Publication: 2024 May 29.
DOI: 10.1016/j.jpba.2024.116242
Abstrakt: The global outbreak of COVID-19 in December 2019 has highlighted rapid and accurate diagnostic tools for effective intervention. While the RT-PCR test offers 86 % sensitivity, uncertainties often require supplementary screening. This research investigates how carbon dots (CDs) can be utilized as markers for COVID-19 antibodies, taking advantage of their biocompatibility and low toxicity. CDs were synthesized using citric acid (CA) and APBA with boronic acid, enabling the detection of COVID-19 IgG antibodies with increased absorbance and fluorescence. Comprehensive analyses confirmed the successful synthesis of APBA-CDs, prompting further exploration of their impact on SARS-CoV-2 RNA. Increased absorbance levels were observed in categories K1, K2, and K3, attributed to the introduction of CDs into plasma, indicating effective binding of APBA-CDs to COVID-19 antibodies. In addition, the fluorescence tests consistently showed heightened levels across all categories, emphasizing the effective binding of APBA-CDs with COVID-19 antibodies, particularly in positive plasma samples. As a part of our analysis, we conducted a PCA test to validate the data, which revealed that APBA-CDs are specific to IgG+ antibodies. The results showed a sensitivity rate of 74 % and a specificity rate of 53 %, while, when tested for IgM antibodies, the sensitivity and specificity rates were 63 % and 27 %, respectively. These findings highlight the potential of APBA-CDs as a sensitive and specific marker for COVID-19 antibody detection, offering potential for diagnostic tool development.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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