Multiplexed neurochemical sensing with sub-nM sensitivity across 2.25 mm 2 area.

Autor: Mintz Hemed N; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA., Hwang FJ; Department of Neurosurgery, Stanford University, Stanford, CA, 94305, USA., Zhao ET; Department of Chemical Engineering, Stanford University, Stanford, CA, 94305, USA., Ding JB; Department of Neurosurgery, Stanford University, Stanford, CA, 94305, USA; Department of Neurology and Neurological Sciences, Stanford University, Stanford, CA, 94305, USA., Melosh NA; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA. Electronic address: nmelosh@stanford.edu.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2024 Oct 01; Vol. 261, pp. 116474. Date of Electronic Publication: 2024 Jun 06.
DOI: 10.1016/j.bios.2024.116474
Abstrakt: Multichannel arrays capable of real-time sensing of neuromodulators in the brain are crucial for gaining insights into new aspects of neural communication. However, measuring neurochemicals, such as dopamine, at low concentrations over large areas has proven challenging. In this research, we demonstrate a novel approach that leverages the scalability and processing power offered by microelectrode array devices integrated with a functionalized, high-density microwire bundle, enabling electrochemical sensing at an unprecedented scale and spatial resolution. The sensors demonstrate outstanding selective molecular recognition by incorporating a selective polymeric membrane. By combining cutting-edge commercial multiplexing, digitization, and data acquisition hardware with a bio-compatible and highly sensitive neurochemical interface array, we establish a powerful platform for neurochemical analysis. This multichannel array has been successfully utilized in vitro and ex vivo systems. Notably, our results show a sensing area of 2.25 mm 2 with an impressive detection limit of 820 pM for dopamine. This new approach paves the way for investigating complex neurochemical processes and holds promise for advancing our understanding of brain function and neurological disorders.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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