HPLC/ESI-QTOF-MS/MS based untargeted metabolomics authentication of Taxus × media six tissues.

Autor: Bai H; College of Forestry, Northwest A&F University, Yangling, Shaanxi, China., Li D; College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.; Shaanxi Key Laboratory of Economic Plant Resources Development and Utilization, Yangling, Shaanxi, China.
Jazyk: angličtina
Zdroj: Phytochemical analysis : PCA [Phytochem Anal] 2024 Oct; Vol. 35 (7), pp. 1600-1612. Date of Electronic Publication: 2024 Jun 13.
DOI: 10.1002/pca.3403
Abstrakt: Introduction: Taxus media (Taxus × media Rehder) is renowned for its high paclitaxel content, serving as a major source for industrial paclitaxel production. In addition to paclitaxel, T. media contains a diverse range of metabolites, including flavonoids, alkaloids, and terpenoids, which have been shown to possess antioxidant, antibacterial, anti-inflammatory, and immunomodulatory effects. However, these compounds have not been thoroughly studied as key metabolites in T. media.
Objective: The untargeted metabolomics analysis of six T. media tissues provides new insights into the development and utilization of T. media metabolites.
Method: The extracts from six tissues of T. media were analyzed and subjected to analysis using high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS/MS) and chemometric techniques.
Results: Using a reliable HPLC-Q-TOF-MS/MS method, we identified 312 compounds in six T. media tissues, including 214 previously unreported in T. media. To identify characteristic compounds across different tissues, 34 metabolites were further screened. KEGG metabolic pathway analysis revealed that these compounds primarily occur in the metabolic pathways of terpene glycosides, flavans, and O-methylated flavonoids.
Conclusion: This study initially utilized an HPLC-QTOF-MS/MS-based metabolomics approach to assess the metabolites in different tissues of T. media, providing a basis for their utilization and management.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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