Ultrasensitive dynamic light scattering immunodetection of alpha-fetoprotein using heptamer-amplified nanoparticle crosslinking aggregation.

Autor: Ding L; Department of Cardiology, The First Affiliated Hospital, Jiangxi Medical College, Jiangxi Hypertension Research Institute, Nanchang University, Nanchang, 330006, P. R. China., Hu J; State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang, 330047, P. R. China.; Songzi Center for Inspection and Test, Songzi, 434200, P. R. China., Liu X; Key Laboratory of Tropical and Vegetables Quality and Safety for State Market Regulation, School of Food Science and Engineering, Hainan University, Haikou, 570228, P. R. China., Zeng J; Department of Cardiology, The First Affiliated Hospital, Jiangxi Medical College, Jiangxi Hypertension Research Institute, Nanchang University, Nanchang, 330006, P. R. China., Hu Z; Department of Cardiology, The First Affiliated Hospital, Jiangxi Medical College, Jiangxi Hypertension Research Institute, Nanchang University, Nanchang, 330006, P. R. China., Chen J; State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang, 330047, P. R. China., Zhu K; State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang, 330047, P. R. China., Duan H; Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University, Beijing, 100048, P. R. China. duanhong202@163.com., Huang X; State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang, 330047, P. R. China. xiaolin.huang@ncu.edu.cn.; Jiangxi Medicine Academy of Nutrition and Health Management, Nanchang, 330006, P. R. China. xiaolin.huang@ncu.edu.cn.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Jun 13; Vol. 191 (7), pp. 387. Date of Electronic Publication: 2024 Jun 13.
DOI: 10.1007/s00604-024-06437-7
Abstrakt: A novel construction strategy is introduced for an ultrasensitive dynamic light scattering (DLS) immunosensor targeting alpha fetoprotein (AFP). This approach relies on a self-assembled heptamer fusion protein (A1-C4bpα), incorporating the dual functions of multivalent recognition and crosslinking aggregation amplification due to the presence of seven AFP-specific A1 nanobodies on the A1-C4bpα heptamer. Leveraging antibody-functionalized magnetic nanoparticles for target AFP capture and DLS signal output, the proposed heptamer-assisted DLS immunosensor offers high sensitivity, strong specificity, and ease of operation. Under the optimized conditions, the designed DLS immunosensor demonstrates excellent linear detection of AFP in the concentration range 0.06 ng mL -1 to 512 ng mL -1 , with a detection limit of 15 pg mL -1 . The selectivity, accuracy, precision, practicability, and reliability of this newly developed method were further validated through an assay of AFP levels in spiked and actual human serum samples. This work introduces a novel approach for constructing ultrasensitive DLS immunosensors, easily extendable to the sensitive determination of other targets via simply replacing the nanobody sequence, holding great promise in various applications, particularly in disease diagnosis.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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