Cyclopropanation and membrane unsaturation improve antibiotic resistance of swarmer Pseudomonas and its sod mutants exposed to radiations, in vitro and in silico approch.

Autor: Kloula Ben Ghorbal S; Laboratoire de Traitement des Eaux Usées, Centre de Recherches et Technologies des Eaux Usées, Technopole Borj Cedria, BP 273, 8020, Soliman, Tunisia. salmakloula@yahoo.fr., Dhaya I; LR18ES03- Laboratory of Neurophysiology Cellular Physiopathology and Biomolecules Valorization, University of Tunis El Manar, Tunis, Tunisia., Ouzari IH; Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Physiques et Naturelles de Tunis, University Tunis El Manar, Tunis, Tunisia., Chatti A; Laboratoire de Traitement des Eaux Usées, Centre de Recherches et Technologies des Eaux Usées, Technopole Borj Cedria, BP 273, 8020, Soliman, Tunisia.; Unite de Biochimie des Lipides et Interactions des Macromolécules en Biologie (03/UR/0902), Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Bizerte, Zarzouna, 7021, Bizerte, Tunisia.
Jazyk: angličtina
Zdroj: World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2024 Jun 13; Vol. 40 (8), pp. 243. Date of Electronic Publication: 2024 Jun 13.
DOI: 10.1007/s11274-024-04033-8
Abstrakt: It was known that UVc irradiation increases the reactive oxygen species' (ROS) levels in bacteria hence the intervention of antioxidant enzymes and causes also changes in fatty acids (FAs) composition enabling bacteria to face antibiotics. Here, we intended to elucidate an interrelationship between SOD and susceptibility to antibiotics by studying FA membrane composition of UVc-treated P. aeruginosa PAO1 and its isogenic mutants (sodM, sodB and sod MB) membrane, after treatment with antibiotics. Swarmer mutants defective in genes encoding superoxide dismutase were pre-exposed to UVc radiations and then tested by disk diffusion method for their contribution to antibiotic tolerance in comparison with the P. aeruginosa wild type (WT). Moreover, fatty acid composition of untreated and UVc-treated WT and sod mutants was examined by Gaz chromatography and correlated to antibiotic resistance. Firstly, it has been demonstrated that after UVc exposure, swarmer WT strain, sodM and sodB mutants remain resistant to polymixin B, a membrane target antibiotic, through membrane unsaturation supported by the intervention of Mn-SOD after short UVc exposure and cyclopropanation of unsaturated FAs supported by the action of Fe-SOD after longer UVc exposure. However, resistance for ciprofloxacin is correlated with increase in saturated FAs. This correlation has been confirmed by a molecular docking approach showing that biotin carboxylase, involved in the initial stage of FA biosynthesis, exhibits a high affinity for ciprofloxacin. This investigation has explored the correlation of antibiotic resistance with FA content of swarmer P.aeruginosa pre-exposed to UVc radiations, confirmed to be antibiotic target dependant.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
Externí odkaz: