Kigelia africana fruit fractions inhibit in vitro alpha-glucosidase activity: a potential natural alpha-glucosidase inhibitor.

Autor:	Muyenga TA; Department of Physiological Sciences, School of Medicine and Health Sciences, Mulungushi University, P.O. Box 60009, Livingstone, Zambia. tumelo.muyenga@mu.ac.zm.; Department of Pharmacology and Therapeutics, Faculty of Health and Veterinary Sciences, University of Namibia, Windhoek, Namibia. tumelo.muyenga@mu.ac.zm., Bamitale SKD; Department of Internal Medicine and Pharmacology, Faculty of Medicine, and Health Sciences, Eastern Cape, Walter Sisulu University, Mthatha, South Africa., Kibuule D; Department of Pharmacology and Therapeutics, Faculty of Health Sciences, Busitema University, Busitema, Uganda., Sithole S; Department of Chemistry and Earth Sciences, University of Zimbabwe, Mt. Pleasant, Harare, Zimbabwe., Mukanganyama S; Department of Biotechnology and Biotechnology, University of Zimbabwe, Mt. Pleasant, Harare, Zimbabwe., Rudolph C; Department of Biochemistry and Microbiology, Nelson Mandela University, Port Elizabeth, South Africa., Venables L; Department of Biochemistry and Microbiology, Nelson Mandela University, Port Elizabeth, South Africa., Hattingh AC; Department of Biochemistry and Microbiology, Nelson Mandela University, Port Elizabeth, South Africa., van de Venter M; Department of Biochemistry and Microbiology, Nelson Mandela University, Port Elizabeth, South Africa., Ezeala CC; College of Health Agriculture and Natural Sciences, Africa University, Mutare, Zimbabwe.
Jazyk:	angličtina
Zdroj:	BMC complementary medicine and therapies [BMC Complement Med Ther] 2024 Jun 12; Vol. 24 (1), pp. 230. Date of Electronic Publication: 2024 Jun 12.
DOI:	10.1186/s12906-024-04510-5
Abstrakt:	Background: Diabetes affects 75% of people in low-income countries, where conventional drugs like metformin are available, but newer drugs like alpha-glucosidase inhibitors are not accessible to most Southern African patients. Aim: To evaluate the α-glucosidase and α-amylase inhibitory activities of fractionated aqueous extracts of Kigelia africana fruit (KAFE) and their phytochemical fingerprints using gas chromatography-mass spectrometry (GC-MS). Materials and Methods: We studied K. africana fruit fractions' inhibitory effects on alpha-glucosidase and alpha-amylase using bioassay-guided fractionation, and analyzed their phytochemical profiles with GC-MS. Key Findings: Both the aqueous extract and ethyl acetate fraction of the aqueous extract exhibited a low dose-dependent inhibition of alpha-amylase activity (p < 0.0001). At a concentration of 500 μg/mL, the aqueous extract caused an alpha-glucosidase inhibition of 64.10 ± 2.7%, with an estimated IC50 of 193.7 μg/mL, while the ethyl acetate fraction had an inhibition of 89.82 ± 0.8% and an estimated IC50 of 10.41 μg/mL. The subfraction G, which had the highest alpha-glucosidase inhibitory activity at 85.10 ± 0.7%, had significantly lower activity than the ethyl acetate fraction. The most bioactive fraction was found to contain 11"(2-cyclopenten-1-yl) undecanoic acid, ( +)- and cyclopentane undecanoic acid as well as the indole alkaloids Akuammilan-17-ol-10-methoxy, N-nitroso-2-methyl-oxazolidine and epoxide Oxirane2.2″ -(1.4-butanediyl) bis-. Conclusion: The K. africana fruit fraction demonstrated significant alpha-glucosidase inhibitory activity, while its alpha-amylase inhibitory activity was limited. This study suggests a potential natural alpha-glucosidase inhibitor and phytocompounds that could serve as leads for developing antidiabetic agents. (© 2024. The Author(s).)
Databáze:	MEDLINE
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