CYP2E1 mediated advanced oxidation protein products exacerbate acetaminophen induced drug-derived liver injury in vitro and in vivo.

Autor: Xun T; Department of Pharmacy, Shenzhen Hospital, Southern Medical University, Shenzhen, China., Zhang M; Department of Pharmacy, Shenzhen Hospital, Southern Medical University, Shenzhen, China., Wei S; Department of Pharmacy, Shenzhen Hospital, Southern Medical University, Shenzhen, China., Zhao C; Department of Pharmacy, Shenzhen Hospital, Southern Medical University, Shenzhen, China., Lin Z; Department of Pharmacy, Shenzhen Hospital, Southern Medical University, Shenzhen, China., Feng H; Department of Neurology, Shenzhen Institute of Translational Medicine, Shenzhen Second People's Hospital. Shenzhen University, Shenzhen, China., Wang X; Department of Pharmacy, Shenzhen Longhua District Central Hospital, Shenzhen, China., Zhao J; Department of Pharmacy, Shenzhen Hospital, Southern Medical University, Shenzhen, China., Yang X; Department of Pharmacy, Shenzhen Hospital, Southern Medical University, Shenzhen, China. Electronic address: yaxx@smu.edu.cn.
Jazyk: angličtina
Zdroj: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences [Eur J Pharm Sci] 2024 Sep 01; Vol. 200, pp. 106829. Date of Electronic Publication: 2024 Jun 10.
DOI: 10.1016/j.ejps.2024.106829
Abstrakt: Drug-induced liver injury (DILI) is prevalent in the treatment of chronic kidney disease (CKD). Advanced oxidation protein products (AOPPs) are markers of CKD progression and participate in the occurrence and development of liver diseases. However, the mechanisms underlying the regulation of DILI in CKD have not been established. Herein, we demonstrate the involvement of Cytochrome p450 2E1 (CYP2E1) in DILI induced by AOPPs is exacerbated by exposure to acetaminophen (APAP). We used a adenine-induced CKD model, a model of DILI induced by APAP, and the AOPPs model was generated by intraperitoneal injection. The decline in renal function was associated with a significantly increased concentration of Scr, BUN and AOPPs, and renal tissue fibrosis. The ALT, AST, and AOPPs levels and liver tissue necrosis increased significantly in CKD model group compared with the sodium carboxymethyl cellulose (CMCNa) group. In the AOPPs model, compared to the PBS controls, ALT, AST, and AOPP levels, and liver tissue necrosis increased significantly. In HepG2 or L0-2 cell lines, cell survival was significantly reduced in the AOPP + APAP treatment and CYP2E1 protein expression was increased. FPS-ZM1 or NAC attenuated the hepatocyte toxicity induced by AOPP + APAP and suppression of CYP2E1 expression. AOPPs exacerbated APAP-induced DILI through CYP2E1 signaling pathways. Protein uremic toxins, such as AOPPs, can modify drug toxicity in patients with CKD. This study provides new a rationale to reduce the generation of DILIs in clinical treatment in patients with CKD. AOPPs targeting may present a novel approach to reduce the occurrence of DILI.
Competing Interests: Conflicts of interest None of the authors have a conflict of interest regarding the present study or have anything to disclose.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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