Sodium fusidate loaded apatitic calcium phosphates: Adsorption behavior, release kinetics, antibacterial efficacy, and cytotoxicity assessment.

Autor: Noukrati H; Cadi Ayyad University, Faculty of Sciences Semlalia (SCIMATOP), Bd Prince My Abdellah, BP 2390, 40000 Marrakech, Morocco; CIRIMAT, Toulouse INP, Université Toulouse 3 Paul Sabatier, CNRS, Université de Toulouse, ENSIACET, 4 Allée Emile Monso, 31030 Toulouse Cedex 4, France; Institute of Biological Sciences, ISSB, Faculty of Medical Sciences (FMS), Mohammed VI Polytechnic University (UM6P), Ben Guerir 43150, Morocco. Electronic address: hassan.noukrati@um6p.ma., Hamdan Y; Institute of Biological Sciences, ISSB, Faculty of Medical Sciences (FMS), Mohammed VI Polytechnic University (UM6P), Ben Guerir 43150, Morocco., Marsan O; CIRIMAT, Toulouse INP, Université Toulouse 3 Paul Sabatier, CNRS, Université de Toulouse, ENSIACET, 4 Allée Emile Monso, 31030 Toulouse Cedex 4, France., El Fatimy R; Institute of Biological Sciences, ISSB, Faculty of Medical Sciences (FMS), Mohammed VI Polytechnic University (UM6P), Ben Guerir 43150, Morocco., Cazalbou S; CIRIMAT, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 118 Route de Narbonne, 31062 Toulouse Cedex 9, France., Rey C; CIRIMAT, Toulouse INP, Université Toulouse 3 Paul Sabatier, CNRS, Université de Toulouse, ENSIACET, 4 Allée Emile Monso, 31030 Toulouse Cedex 4, France., Barroug A; Cadi Ayyad University, Faculty of Sciences Semlalia (SCIMATOP), Bd Prince My Abdellah, BP 2390, 40000 Marrakech, Morocco; Institute of Biological Sciences, ISSB, Faculty of Medical Sciences (FMS), Mohammed VI Polytechnic University (UM6P), Ben Guerir 43150, Morocco., Combes C; CIRIMAT, Toulouse INP, Université Toulouse 3 Paul Sabatier, CNRS, Université de Toulouse, ENSIACET, 4 Allée Emile Monso, 31030 Toulouse Cedex 4, France.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2024 Jul 20; Vol. 660, pp. 124331. Date of Electronic Publication: 2024 Jun 10.
DOI: 10.1016/j.ijpharm.2024.124331
Abstrakt: The present work reports the adsorption, release, antibacterial properties, and in vitro cytotoxicity of sodium fusidate (SF) associated with a carbonated calcium phosphate bone cement. The adsorption study of SF on cement powder compared to stoichiometric hydroxyapatite and nanocrystalline carbonated apatite was investigated to understand the interaction between this antibiotic and the calcium phosphate phases involved in the cement formulation and setting reaction. The adsorption data revealed a fast kinetic process. However, the evolution of the amount of adsorbed SF was well described by a Freundlich-type isotherm characterized by a low adsorption capacity of the materials toward the SF molecule. The in vitro release results indicated a prolonged and controlled SF release for up to 34 days. The SF amounts eluted daily were at a therapeutic level (0.5-2 mg/L) and close to the antibiotic minimum inhibitory concentration (0.1-0.9 mg/L). Furthermore, the release data fitting and modeling suggested that the drug release occurred mainly by a diffusion mechanism. The antibacterial activity showed the effectiveness of SF released from the formulated cements against Staphylococcus aureus. Furthermore, the biological in vitro study demonstrated that the tested cements didn't show any cytotoxicity towards human peripheral blood mononuclear cells and did not significantly induce inflammation markers like IL-8.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: