A growth selection system for sucrose synthases (SuSy): design and test.

Autor: Bidart GN; The Novo Nordisk Center for Biosustainability, Technical University of Denmark, Kemitorvet 220, Kgs. Lyngby, DK-2800, Denmark., Hyeuk S; The Novo Nordisk Center for Biosustainability, Technical University of Denmark, Kemitorvet 220, Kgs. Lyngby, DK-2800, Denmark., Alter TB; The Novo Nordisk Center for Biosustainability, Technical University of Denmark, Kemitorvet 220, Kgs. Lyngby, DK-2800, Denmark.; RWTH Aachen University, Templergraben 55, 52062, Aachen, Germany., Yang L; The Novo Nordisk Center for Biosustainability, Technical University of Denmark, Kemitorvet 220, Kgs. Lyngby, DK-2800, Denmark., Welner DH; The Novo Nordisk Center for Biosustainability, Technical University of Denmark, Kemitorvet 220, Kgs. Lyngby, DK-2800, Denmark. diwel@biosustain.dtu.dk.
Jazyk: angličtina
Zdroj: AMB Express [AMB Express] 2024 Jun 12; Vol. 14 (1), pp. 70. Date of Electronic Publication: 2024 Jun 12.
DOI: 10.1186/s13568-024-01727-y
Abstrakt: High throughput screening (HTS) methods of enzyme variants are essential for the development of robust biocatalysts suited for low impact, industrial scale, biobased synthesis of a myriad of compounds. However, for the majority of enzyme classes, current screening methods have limited throughput, or need expensive substrates in combination with sophisticated setups. Here, we present a straightforward, high throughput selection system that couples sucrose synthase activity to growth. Enabling high throughput screening of this enzyme class holds the potential to facilitate the creation of robust variants, which in turn can significantly impact the future of cost effective industrial glycosylation.
(© 2024. The Author(s).)
Databáze: MEDLINE
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