Arsenic stress triggers active exudation of arsenic-phytochelatin complexes from Lupinus albus roots.

Autor:	Frémont A; Institut de recherche en biologie végétale, Département de sciences biologiques, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC H1X 2B2, Canada.; Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA., Sas E; Institut de recherche en biologie végétale, Département de sciences biologiques, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC H1X 2B2, Canada., Sarrazin M; Collège de Maisonneuve CÉPROCQ, 6220 Sherbrooke Est, Montréal, QC H1N 1C1, Canada., Brisson J; Institut de recherche en biologie végétale, Département de sciences biologiques, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC H1X 2B2, Canada., Pitre FE; Institut de recherche en biologie végétale, Département de sciences biologiques, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC H1X 2B2, Canada.; Montreal Botanical Garden, 4101 Sherbrooke Est, Montreal, QC H1X 2B2, Canada., Brereton NJB; School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland.
Jazyk:	angličtina
Zdroj:	Journal of experimental botany [J Exp Bot] 2024 Sep 27; Vol. 75 (18), pp. 5897-5908.
DOI:	10.1093/jxb/erae272
Abstrakt:	Arsenic (As) contamination of soils threatens the health of millions globally through accumulation in crops. While plants detoxify As via phytochelatin (PC) complexation and efflux of arsenite from roots, arsenite efflux mechanisms are not fully understood. Here, white lupin (Lupinus albus) was grown in semi-hydroponics, and exudation of glutathione (GSH) derivatives and PCs in response to As was measured using LC-MS/MS. Inhibiting synthesis of the PC precursor GSH with l-buthionine sulfoximine (BSO) or ABC transporters with vanadate drastically reduced (>22%) GSH derivative and PC2 exudation, but not PC3 exudation. This was accompanied by As hypersensitivity in plants treated with BSO and moderate sensitivity with vanadate treatment. Investigating As-PC complexation revealed two distinct As-PC complexes, As bound to GSH and PC2 (GS-As-PC2) and As bound to PC3 (As-PC3), in exudates of As-treated lupin plants. Vanadate inhibited As-PC exudation, while BSO inhibited both the synthesis and exudation of As-PC complexes. These results demonstrate a role for GSH derivatives and PC exudation in lupin As tolerance and reveal As-PC exudation as a new potential mechanism contributing to active As efflux in plants. Overall, this study uncovers insights into rhizosphere As detoxification with potential to help mitigate pollution and reduce As accumulation in crops. (© The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology.)
Databáze:	MEDLINE
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