Serotype and genomic diversity of dengue virus during the 2023 outbreak in Pakistan reveals the circulation of genotype III of DENV-1 and cosmopolitan genotype of DENV-2.

Autor: Jamal Z; National Institutes of Health, Islamabad, Pakistan., Haider SA; National Institutes of Health, Islamabad, Pakistan., Hakim R; National Institutes of Health, Islamabad, Pakistan., Humayun F; Jhpiego, Islamabad, Pakistan., Farooq MU; Jhpiego, Islamabad, Pakistan., Ammar M; National Institutes of Health, Islamabad, Pakistan., Afrough B; New Variant Assessment Platform, UK Health Security Agency, London, UK., Inamdar L; New Variant Assessment Platform, UK Health Security Agency, London, UK., Salman M; National Institutes of Health, Islamabad, Pakistan., Umair M; National Institutes of Health, Islamabad, Pakistan.
Jazyk: angličtina
Zdroj: Journal of medical virology [J Med Virol] 2024 Jun; Vol. 96 (6), pp. e29727.
DOI: 10.1002/jmv.29727
Abstrakt: Dengue, a mosquito-borne viral disease, poses a significant public health challenge in Pakistan, with a significant outbreak in 2023, prompting our investigation into the serotype and genomic diversity of the dengue virus (DENV). NS-1 positive blood samples from 153 patients were referred to the National Institute of Health, Pakistan, between July and October 2023. Among these, 98 (64.1%) tested positive using multiplex real-time PCR, with higher prevalence among males (65.8%) and individuals aged 31-40. Serotyping revealed DENV-1 as the predominant serotype (84.7%), followed by DENV-2 (15.3%). Whole-genome sequencing of 18 samples (DENV-1 = 17, DENV-2 = 01) showed that DENV-1 (genotype III) samples were closely related (>99%) to Pakistan outbreak samples (2022), and approx. > 98% with USA (2022), Singapore and China (2016), Bangladesh (2017), and Pakistan (2019). The DENV-2 sequence (cosmopolitan genotype; clade IVA) shared genetic similarity with Pakistan outbreak sequences (2022), approx. > 99% with China and Singapore (2018-2019) and showed divergence from Pakistan sequences (2008-2013). No coinfection with dengue serotypes or other viruses were observed. Comparisons with previous DENV-1 sequences highlighted genetic variations affecting viral replication efficiency (NS2B:K55R) and infectivity (E:M272T). These findings contribute to dengue epidemiology understanding and underscore the importance of ongoing genomic surveillance for future outbreak responses in Pakistan.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: