| Autor: |
Ramos-Llorens M; Instituto de Acuicultura Torre de la Sal (IATS), CSIC , Ribera de Cabanes, Castellón 12595, Spain., Bainour K; Instituto de Acuicultura Torre de la Sal (IATS), CSIC , Ribera de Cabanes, Castellón 12595, Spain., Adelmann L; Max Perutz Labs, University of Vienna , Vienna 1030, Austria.; Research Platform 'Rhythms of Life', University of Vienna, Vienna BioCenter, Dr. Bohr Gasse 9/4, A-1030 , Vienna 1030, Austria., Hontoria F; Instituto de Acuicultura Torre de la Sal (IATS), CSIC , Ribera de Cabanes, Castellón 12595, Spain., Navarro JC; Instituto de Acuicultura Torre de la Sal (IATS), CSIC , Ribera de Cabanes, Castellón 12595, Spain., Raible F; Max Perutz Labs, University of Vienna , Vienna 1030, Austria.; Research Platform 'Rhythms of Life', University of Vienna, Vienna BioCenter, Dr. Bohr Gasse 9/4, A-1030 , Vienna 1030, Austria., Monroig Ó; Instituto de Acuicultura Torre de la Sal (IATS), CSIC , Ribera de Cabanes, Castellón 12595, Spain. |
| Abstrakt: |
Elongation of very long-chain fatty acid (Elovl) proteins plays pivotal functions in the biosynthesis of the physiologically essential long-chain polyunsaturated fatty acids (LC-PUFA). Polychaetes have important roles in marine ecosystems, contributing not only to nutrient recycling but also exhibiting a distinctive capacity for biosynthesizing LC-PUFA. To expand our understanding of the LC-PUFA biosynthesis in polychaetes, this study conducted a thorough molecular and functional characterization of Elovl occurring in the model organism Platynereis dumerilii . We identify six Elovl in the genome of P. dumerilii . The sequence and phylogenetic analyses established that four Elovl, identified as Elovl2/5, Elovl4 (two genes) and Elovl1/7, have putative functions in LC-PUFA biosynthesis. Functional characterization confirmed the roles of these elongases in LC-PUFA biosynthesis, demonstrating that P. dumerilii possesses a varied and functionally diverse complement of Elovl that, along with the enzymatic specificities of previously characterized desaturases, enables P. dumerilii to perform all the reactions required for the biosynthesis of the LC-PUFA. Importantly, we uncovered that one of the two Elovl4-encoding genes is remarkably long in comparison with any other animals' Elovl, which contains a C terminal KH domain unique among Elovl. The distinctive expression pattern of this protein in photoreceptors strongly suggests a central role in vision. |
