Association between matrix metalloproteinase-9-1562C/T gene polymorphism and MMP-9 serum level in rheumatoid arthritis.

Autor:	Foroughi F; Cellular and Molecular Research Center, Research Institute for Prevention of Non-Communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran.; Department of Immunology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.; USERN Office, Qazvin University of Medical Science, Qazvin, Iran., Keshavarz Sadegh R; USERN Office, Qazvin University of Medical Science, Qazvin, Iran.; Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran., Khalaji M; USERN Office, Qazvin University of Medical Science, Qazvin, Iran.; Department of Biochemistry & Genetics, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran., Lashgari M; USERN Office, Qazvin University of Medical Science, Qazvin, Iran.; Metabolic Disease Research Center, Research Institute for prevention of non-Communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran., Javadi A; USERN Office, Qazvin University of Medical Science, Qazvin, Iran.; Medical informatics, Department of Community Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran., Sahmani M; USERN Office, Qazvin University of Medical Science, Qazvin, Iran.; Department of Biochemistry & Genetics, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran., Nonejad S; USERN Office, Qazvin University of Medical Science, Qazvin, Iran.; Department of Biochemistry & Genetics, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran., Keshavarz Shahbaz S; Cellular and Molecular Research Center, Research Institute for Prevention of Non-Communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran.; USERN Office, Qazvin University of Medical Science, Qazvin, Iran.
Jazyk:	angličtina
Zdroj:	Journal of immunoassay & immunochemistry [J Immunoassay Immunochem] 2024 Jul 03; Vol. 45 (4), pp. 362-381. Date of Electronic Publication: 2024 Jun 11.
DOI:	10.1080/15321819.2024.2365699
Abstrakt:	Background: Rheumatoid arthritis (RA) is an autoimmune disease indicated by joint inflammation and cartilage destruction. Matrix metalloproteinase (MMP) enzymes play an influential role in inflammation by affecting the invasion and degradation of anatomical barriers. In this way, the current study investigated the relationship between the MMP-9-1562C/T gene polymorphism and this enzyme's serum level in RA. Methods: The serum levels of MMP-9 in RA patients and healthy controls were measured using the enzyme-linked immunosorbent assay (ELISA). RA was confirmed using rheumatoid factor (RF), anti-cyclic citrullinated peptide (anti-CCP), and C-reactive protein (CRP). Then the MMP-9-1562C/T gene polymorphism was analyzed utilizing polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Also, multivariate analysis investigated the connection between this polymorphism and the risk of RA. Results: In this study, the increase of MMP-9 in patients due to the development of single nucleotide polymorphism in the promoter region of this gene (-1562 C→T) was confirmed by increasing the frequency of heterozygous genotype (CT). Logistic regression analysis also demonstrated that the chance of development of RA is higher in people with CT/CC genotype than in other alleles. Conclusions: We demonstrated that MMP-9-1562C/T gene polymorphism can play a significant role in the occurrence of RA.
Databáze:	MEDLINE
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