BATF promotes tumor progression and association with FDG PET-derived parameters in colorectal cancer.
Autor: | Lu X; Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China., Liu J; Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China., Feng L; Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China., Huang Y; Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, 226001, China., Xu Y; Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China., Li C; Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China., Wang W; Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China., Kan Y; Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China., Yang J; Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China. yangjigang@ccmu.edu.cn., Zhang M; Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China. zhangmingyu_118@163.com. |
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Jazyk: | angličtina |
Zdroj: | Journal of translational medicine [J Transl Med] 2024 Jun 11; Vol. 22 (1), pp. 558. Date of Electronic Publication: 2024 Jun 11. |
DOI: | 10.1186/s12967-024-05367-5 |
Abstrakt: | Purpose: The purpose of the study was to evaluate the expression and function of basic leucine zipper ATF-like transcription factor (BATF) in colorectal cancer (CRC), and its correlation with 2-deoxy-2[ 18 F]fluoro-D-glucose ( 18 F-FDG) positron emission tomography/computed tomography (PET/CT) parameters. Methods: The TIMER database, GEPIA database, TCGA, and GEO database were used to analyze the expression profile of BATF in human cancers. The reverse transcription‑quantitative PCR and western blot analyses were used to evaluate the mRNA level and protein expression in different CRC cell lines. The expression of BATF in SW620 and HCT116 cells was silenced and cell counting kit-8 assays and clonogenic assay were utilized to evaluate the role of BATF in CRC proliferation. The expression of tumor BATF and glucose transporter 1 (GLUT-1) were examined using immunohistochemical tools in 37 CRC patients undergoing preoperative 18 F-FDG PET/CT imaging. The correlation between the PET/CT parameters and immunohistochemical result was evaluated. Results: In database, BATF was highly expressed in pan-cancer analyses, including CRC, and was associated with poor prognosis in CRC. In vitro, the results showed that knocking down of BATF expression could inhibit the proliferation of SW620 and HCT116 cells. In CRC patients, BATF expression was upregulated in tumor tissues compared with matched para-tumoral tissues, and was related with gender and Ki-67 levels. BATF expression was positively related to GLUT-1 expression and PET/CT parameters, including tumor size, maximum standard uptake value, metabolic tumor volume, and total lesion glycolysis. The multiple logistic analyses showed that SUV Conclusion: BATF may be an oncogene associated with 18 F-FDG PET/CT parameters in CRC. SUV (© 2024. The Author(s).) |
Databáze: | MEDLINE |
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