The nuclear and mitochondrial genome assemblies of Tetragonisca angustula (Apidae: Meliponini), a tiny yet remarkable pollinator in the Neotropics.

Autor: Ferrari RR; Centro de Formação em Ciências Ambientais, Universidade Federal do Sul da Bahia, Porto Seguro, Brazil.; Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China., Ricardo PC; Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil., Dias FC; Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil., de Souza Araujo N; Evolutionary Biology & Ecology, Université Libre de Bruxelles, Bruxelles, Belgium., Soares DO; Centro de Formação em Ciências Ambientais, Universidade Federal do Sul da Bahia, Porto Seguro, Brazil., Zhou QS; Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China., Zhu CD; Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.; Sate Key Laboratory of Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing, China., Coutinho LL; Departamento de Ciências Animais, Universidade de São Paulo/ESALQ, Piracicaba, Brazil., Arias MC; Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. mcarias@ib.usp.br., Batista TM; Centro de Formação em Ciências Ambientais, Universidade Federal do Sul da Bahia, Porto Seguro, Brazil. thiagomafra@ufsb.edu.br.
Jazyk: angličtina
Zdroj: BMC genomics [BMC Genomics] 2024 Jun 11; Vol. 25 (1), pp. 587. Date of Electronic Publication: 2024 Jun 11.
DOI: 10.1186/s12864-024-10502-z
Abstrakt: Background: The field of bee genomics has considerably advanced in recent years, however, the most diverse group of honey producers on the planet, the stingless bees, are still largely neglected. In fact, only eleven of the ~ 600 described stingless bee species have been sequenced, and only three using a long-read (LR) sequencing technology. Here, we sequenced the nuclear and mitochondrial genomes of the most common, widespread and broadly reared stingless bee in Brazil and other neotropical countries-Tetragonisca angustula (popularly known in Brazil as jataí).
Results: A total of 48.01 Gb of DNA data were generated, including 2.31 Gb of Pacific Bioscience HiFi reads and 45.70 Gb of Illumina short reads (SRs). Our preferred assembly comprised 683 contigs encompassing 284.49 Mb, 62.84 Mb of which (22.09%) corresponded to 445,793 repetitive elements. N50, L50 and complete BUSCOs reached 1.02 Mb, 91 contigs and 97.1%, respectively. We predicted that the genome of T. angustula comprises 17,459 protein-coding genes and 4,108 non-coding RNAs. The mitogenome consisted of 17,410 bp, and all 37 genes were found to be on the positive strand, an unusual feature among bees. A phylogenomic analysis of 26 hymenopteran species revealed that six odorant receptor orthogroups of T. angustula were found to be experiencing rapid evolution, four of them undergoing significant contractions.
Conclusions: Here, we provided the first nuclear and mitochondrial genome assemblies for the ecologically and economically important T. angustula, the fourth stingless bee species to be sequenced with LR technology thus far. We demonstrated that even relatively small amounts of LR data in combination with sufficient SR data can yield high-quality genome assemblies for bees.
(© 2024. The Author(s).)
Databáze: MEDLINE
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