Phytate metabolism is mediated by microbial cross-feeding in the gut microbiota.

Autor: De Vos WM; Laboratory of Microbiology, Wageningen University, Wageningen, the Netherlands., Nguyen Trung M; Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.; Institute of Chemistry, Humboldt-Universität zu Berlin, Berlin, Germany., Davids M; Departments of Internal and Experimental Vascular Medicine, Amsterdam University Medical Center, Location AMC, Amsterdam, the Netherlands., Liu G; Institute of Organic Chemistry & Centre for Integrative Biological Signaling Studies (CIBSS), University of Freiburg, Freiburg, Germany., Rios-Morales M; Departments of Internal and Experimental Vascular Medicine, Amsterdam University Medical Center, Location AMC, Amsterdam, the Netherlands., Jessen H; Institute of Organic Chemistry & Centre for Integrative Biological Signaling Studies (CIBSS), University of Freiburg, Freiburg, Germany., Fiedler D; Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.; Institute of Chemistry, Humboldt-Universität zu Berlin, Berlin, Germany., Nieuwdorp M; Departments of Internal and Experimental Vascular Medicine, Amsterdam University Medical Center, Location AMC, Amsterdam, the Netherlands.; Department of Surgery, Spaarne Hospital, Hoofddorp, the Netherlands., Bui TPN; Laboratory of Microbiology, Wageningen University, Wageningen, the Netherlands. t.p.n.bui@amsterdamumc.nl.; Departments of Internal and Experimental Vascular Medicine, Amsterdam University Medical Center, Location AMC, Amsterdam, the Netherlands. t.p.n.bui@amsterdamumc.nl.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2024 Jul; Vol. 9 (7), pp. 1812-1827. Date of Electronic Publication: 2024 Jun 10.
DOI: 10.1038/s41564-024-01698-7
Abstrakt: Dietary intake of phytate has various reported health benefits. Previous work showed that the gut microbiota can convert phytate to short-chain fatty acids (SCFAs), but the microbial species and metabolic pathway are unclear. Here we identified Mitsuokella jalaludinii as an efficient phytate degrader, which works synergistically with Anaerostipes rhamnosivorans to produce the SCFA propionate. Analysis of published human gut taxonomic profiles revealed that Mitsuokella spp., in particular M. jalaludinii, are prevalent in human gut microbiomes. NMR spectroscopy using 13 C-isotope labelling, metabolomic and transcriptomic analyses identified a complete phytate degradation pathway in M. jalaludinii, including production of the intermediate Ins(2)P/myo-inositol. The major end product, 3-hydroxypropionate, was converted into propionate via a synergistic interaction with Anaerostipes rhamnosivorans both in vitro and in mice. Upon [ 13 C 6 ]phytate administration, various 13 C-labelled components were detected in mouse caecum in contrast with the absence of [ 13 C 6 ] InsPs or [ 13 C 6 ]myo-inositol in plasma. Caco-2 cells incubated with co-culture supernatants exhibited improved intestinal barrier integrity. These results suggest that the microbiome plays a major role in the metabolism of this phytochemical and that its fermentation to propionate by M. jalaludinii and A. rhamnosivorans may contribute to phytate-driven health benefits.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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