Genome-wide association study revealed significant SNPs for anthracnose resistance, seed alkaloids and protein content in white lupin.

Autor: Schwertfirm G; Bavarian State Research Center for Agriculture (LfL), Institute for Crop Science and Plant Breeding, Am Gereuth 2, 85354, Freising, Germany. grit.schwertfirm@lfl.bayern.de., Schneider M; Department of Crop Sciences, Research Institute of Organic Agriculture (FiBL), Ackerstrasse 113, Box 219, 5070, Frick, Switzerland., Haase F; Federal Research Centre for Cultivated Plants, Institute for Breeding Research On Agricultural Crops, Julius Kuehn-Institute (JKI), Rudolf Schick Platz 3a, 18190, Groß Lüsewitz, Germany., Riedel C; Bavarian State Research Center for Agriculture (LfL), Institute for Crop Science and Plant Breeding, Kleeberg 14, 94099, Ruhstorf a. d. Rott, Germany., Lazzaro M; Department of Crop Sciences, Research Institute of Organic Agriculture (FiBL), Ackerstrasse 113, Box 219, 5070, Frick, Switzerland., Ruge-Wehling B; Federal Research Centre for Cultivated Plants, Institute for Breeding Research On Agricultural Crops, Julius Kuehn-Institute (JKI), Rudolf Schick Platz 3a, 18190, Groß Lüsewitz, Germany., Schweizer G; Bavarian State Research Center for Agriculture (LfL), Institute for Crop Science and Plant Breeding, Am Gereuth 2, 85354, Freising, Germany.
Jazyk: angličtina
Zdroj: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2024 Jun 10; Vol. 137 (7), pp. 155. Date of Electronic Publication: 2024 Jun 10.
DOI: 10.1007/s00122-024-04665-2
Abstrakt: White lupin (Lupinus albus L.) is a high-protein grain legume alternative to soybean in Central Europe, but its cultivation is risky due to the fungal disease anthracnose that can cause severe yield damage. In addition, management of seed alkaloids is critical for human nutrition and animal feed. We report on a white lupin collection of genebank accessions, advanced breeding lines and cultivars that was genotyped and phenotypically characterized for anthracnose resistance and seed alkaloids and protein levels. Using genotyping by sequencing (GBS), SeqSNP-targeted GBS, BiomarkX genotyping and Sanger sequencing, a genetic resource of genome-wide SNPs for white lupin was established. We determined anthracnose resistance in two years field trials at four locations with infection rows and measured seed alkaloids and protein levels by near-infrared spectroscopy (NIRS). Few white lupin breeding lines showed anthracnose resistance comparable or better than Celina and Frieda, currently the best commercial cultivars in Germany. NIRS estimates for seed alkaloids and protein levels revealed variation in the white lupin collection. Using genome-wide association studies (GWAS), we identified SNPs significantly associated with anthracnose resistance in the field representing known and new genomic regions. We confirmed the pauper locus and detected new SNP markers significantly associated with seed alkaloids. For the first time, we present loci associated with total grain protein content. Finally, we tested the potential of genomic prediction (GP) in predicting the phenotype of these three quantitative traits. Application of results and resources are discussed in the context of fostering breeding programs for white lupin.
(© 2024. The Author(s).)
Databáze: MEDLINE
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