The nucleolar phase of signal recognition particle assembly.

Autor:	Issa A; https://ror.org/04vfs2w97 Université de Lorraine, CNRS, IMoPA, Nancy, France., Schlotter F; https://ror.org/04vfs2w97 Université de Lorraine, CNRS, IMoPA, Nancy, France., Flayac J; https://ror.org/04vfs2w97 Université de Lorraine, CNRS, IMoPA, Nancy, France., Chen J; RNA Molecular Biology, Fonds de la Recherche Scientifique (F.R.S./FNRS), Université libre de Bruxelles (ULB), Charleroi-Gosselies, Belgium., Wacheul L; RNA Molecular Biology, Fonds de la Recherche Scientifique (F.R.S./FNRS), Université libre de Bruxelles (ULB), Charleroi-Gosselies, Belgium., Philippe M; IGH, University Montpellier, CNRS, Montpellier, France., Sardini L; https://ror.org/04vfs2w97 Université de Lorraine, CNRS, IMoPA, Nancy, France., Mostefa L; https://ror.org/04vfs2w97 Université de Lorraine, CNRS, IMoPA, Nancy, France., Vandermoere F; IGF, University Montpellier, CNRS, INSERM, Montpellier, France., Bertrand E; IGH, University Montpellier, CNRS, Montpellier, France., Verheggen C; IGH, University Montpellier, CNRS, Montpellier, France celine.verheggen@igh.cnrs.fr., Lafontaine DL; RNA Molecular Biology, Fonds de la Recherche Scientifique (F.R.S./FNRS), Université libre de Bruxelles (ULB), Charleroi-Gosselies, Belgium denis.lafontaine@ulb.be., Massenet S; https://ror.org/04vfs2w97 Université de Lorraine, CNRS, IMoPA, Nancy, France severine.massenet@univ-lorraine.fr.
Jazyk:	angličtina
Zdroj:	Life science alliance [Life Sci Alliance] 2024 Jun 10; Vol. 7 (8). Date of Electronic Publication: 2024 Jun 10 (Print Publication: 2024).
DOI:	10.26508/lsa.202402614
Abstrakt:	The signal recognition particle is essential for targeting transmembrane and secreted proteins to the endoplasmic reticulum. Remarkably, because they work together in the cytoplasm, the SRP and ribosomes are assembled in the same biomolecular condensate: the nucleolus. How important is the nucleolus for SRP assembly is not known. Using quantitative proteomics, we have investigated the interactomes of SRP components. We reveal that SRP proteins are associated with scores of nucleolar proteins important for ribosome biogenesis and nucleolar structure. Having monitored the subcellular distribution of SRP proteins upon controlled nucleolar disruption, we conclude that an intact organelle is required for their proper localization. Lastly, we have detected two SRP proteins in Cajal bodies, which indicates that previously undocumented steps of SRP assembly may occur in these bodies. This work highlights the importance of a structurally and functionally intact nucleolus for efficient SRP production and suggests that the biogenesis of SRP and ribosomes may be coordinated in the nucleolus by common assembly factors. (© 2024 Issa et al.)
Databáze:	MEDLINE
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