Differential distribution of characteristic constituents in peel and pulp of Aurantii Fructus Immaturus (Citrus aurantium L.) using MALDI mass spectrometry imaging.

Autor: Cai W; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China; Department of Pharmacy, Peking University First Hospital, Beijing 100034, China., Zhang S; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China., Wang Y; School of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China., Liu C; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China., Luo R; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. Electronic address: luorong@ccmu.edu.cn.
Jazyk: angličtina
Zdroj: Fitoterapia [Fitoterapia] 2024 Sep; Vol. 177, pp. 106067. Date of Electronic Publication: 2024 Jun 08.
DOI: 10.1016/j.fitote.2024.106067
Abstrakt: Aurantii Fructus Immaturus (AFI) was structurally divided into two parts named "peel" and "pulp". The exocarp and mesocarp of materials named "peel". The endocarp separated into multiple compartments and the cystic hair attached to it named "pulp". In order to explore the distribution and content of constituents in AFI, an efficient method to explore the distribution of constituents was developed based on matrix assisted laser desorption/ionization fourier transform ion cyclotron resonance mass spectrometry imaging (MALDI-FTICR-MSI). After simple processing, thirty-two constituents with distinct localization in the mass range of 101-1200 Da were identified by MALDI-FTICR-MSI. In addition, the identified four flavnoids (poncirin, sinensetin, 3,5,6,7,8,3',4'-heptemthoxyflavone, and tangeritin) were analyzed for differences between using LC-MS. Quantitative analysis results supported the quantitative results from MALDI-FT-ICR-MSI. The results implied that different parts had different constituents in AFI, and demonstrated MALDI-MSI have high potential in the direct analysis of constituents.
Competing Interests: Declaration of competing interest The authors do not have any conflict of interests.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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