Development and characterization of polyethylene oxide and guar gum-based hydrogel; a detailed in-vitro analysis of degradation and drug release kinetics.

Autor: Aizaz A; Centre of Excellence in Biomaterials and Tissue Engineering, Department of Materials Science and Engineering Government College University Lahore, 54000, Pakistan., Nawaz MH; Department of Materials Science & Engineering, Institute of Space Technology Islamabad, 1, Islamabad Highway, Islamabad, 44000, Pakistan., Ismat MS; Department of Materials Science & Engineering, Institute of Space Technology Islamabad, 1, Islamabad Highway, Islamabad, 44000, Pakistan., Zahid L; Department of Materials Science & Engineering, Institute of Space Technology Islamabad, 1, Islamabad Highway, Islamabad, 44000, Pakistan., Zahid S; Department of Materials Science & Engineering, Institute of Space Technology Islamabad, 1, Islamabad Highway, Islamabad, 44000, Pakistan., Ahmed S; Department of Materials Science & Engineering, Institute of Space Technology Islamabad, 1, Islamabad Highway, Islamabad, 44000, Pakistan., Abbas M; Central Labs, King Khalid University, AlQura'a, Abha, P.O. Box 960, Saudi Arabia; Electrical Engineering Department, College of Engineering, King Khalid University, Abha 61421, Saudi Arabia., Vayalpurayil T; Central Labs, King Khalid University, AlQura'a, Abha, P.O. Box 960, Saudi Arabia; Electrical Engineering Department, College of Engineering, King Khalid University, Abha 61421, Saudi Arabia., Rehman MAU; Centre of Excellence in Biomaterials and Tissue Engineering, Department of Materials Science and Engineering Government College University Lahore, 54000, Pakistan; Department of Materials Science & Engineering, Institute of Space Technology Islamabad, 1, Islamabad Highway, Islamabad, 44000, Pakistan. Electronic address: atique1.1@hotmail.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Jul; Vol. 273 (Pt 1), pp. 132824. Date of Electronic Publication: 2024 Jun 08.
DOI: 10.1016/j.ijbiomac.2024.132824
Abstrakt: Herein, we synthesized hydrogel films from crosslinked polyethylene oxide (PEO) and guar gum (GG) which can offer hydrophilicity, antibacterial efficacy, and neovascularization. This study focuses on synthesis and material/biological characterization of rosemary (RM) and citric acid (CA) loaded PEO/GG hydrogel films. Scanning Electron Microscopy images confirmed the porous structure of the developed hydrogel film matrix (PEO/GG) and the dispersion of RM and CA within it. This porous structure promotes moisture adsorption, cell attachment, proliferation, and tissue layer formation. Fourier Transform Infrared Spectroscopy (FTIR) further validated the crosslinking of the PEO/GG matrix, as confirmed by the appearance of C-O-C linkage in the FTIR spectrum. PEO/GG and PEO/GG/RM/CA revealed similar degradation and release kinetics in Dulbecco's Modified Eagle Medium, Simulated Body Fluid, and Phosphate Buffer Saline (degradation of ∼55 % and release of ∼60 % RM in 168 h.). The developed hydrogel film exhibited a zone of inhibition against Escherichia. coli (2 mm) and Staphylococcus. aureus (9 mm), which can be attributed to the presence of RM in the hydrogel film. Furthermore, incorporating CA in the hydrogel film promoted neovascularization, as confirmed by the Chorioallantoic Membrane Assay. The developed RM and CA-loaded PEO/GG-based hydrogel films offered suitable in-vitro properties that may aid in potential wound healing applications.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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Databáze: MEDLINE
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