Vaccine efficacy induced by virus-like particles containing Leishmania donovani surface glycoprotein GP63.

Autor: Yoon KW; Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Republic of Korea., Chu KB; Department of Parasitology, Inje University College of Medicine, Busan, Republic of Korea.; Department of Infectious Disease and Malaria, Paik Institute of Clinical Research, Inje University, Busan, Republic of Korea., Eom GD; Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Republic of Korea., Mao J; Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Republic of Korea., Quan FS; Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, Core Research Institute (CRI), Kyung Hee University, Seoul, Republic of Korea.; Department of Medical Zoology, School of Medicine, Kyung Hee University, Seoul, Republic of Korea.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2024 Jun 10; Vol. 18 (6), pp. e0012229. Date of Electronic Publication: 2024 Jun 10 (Print Publication: 2024).
DOI: 10.1371/journal.pntd.0012229
Abstrakt: Leishmania donovani surface glycoprotein 63 (GP63) is a major virulence factor involved in parasite escape and immune evasion. In this study, we generated virus-like particles (VLPs) expressing L. donovani GP63 using the baculovirus expression system. Mice were intramuscularly immunized with GP63-VLPs and challenged with L. donovani promastigotes. GP63-VLP immunization elicited higher levels of L. donovani antigen-specific serum antibodies and enhanced splenic B cell, germinal center B cell, CD4+, and CD8+ T cell responses compared to unimmunized controls. GP63-VLPs inhibited the influx of pro-inflammatory cytokines IFN-γ and IL-6 in the livers, as well as thwarting the development of splenomegaly in immunized mice. Upon L. donovani challenge infection, a drastic reduction in splenic parasite burden was observed in VLP-immunized mice. These results indicate that GP63-VLPs immunization conferred protection against L. donovani challenge infection by inducing humoral and cellular immunity in mice.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Yoon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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