Bioproducts of Pseudomonas chlororaphis Suppress DMI Fungicide-Induced CsCYP51A and CsCYP51B Gene Expression in Colletotrichum siamense and Generate Synergistic Effects with Metconazole and Propiconazole.
| Autor: | Wesche J; Department of Plant and Environmental Science, Clemson University, 105 Collings Street, Clemson, SC 29634, U.S.A., Wu P; Hubei Key Lab of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China., Luo CX; Hubei Key Lab of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China., Faust JE; Department of Plant and Environmental Science, Clemson University, 105 Collings Street, Clemson, SC 29634, U.S.A., Schnabel G; Department of Plant and Environmental Science, Clemson University, 105 Collings Street, Clemson, SC 29634, U.S.A. |
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| Jazyk: | angličtina |
| Zdroj: | Phytopathology [Phytopathology] 2024 Sep; Vol. 114 (9), pp. 2064-2070. Date of Electronic Publication: 2024 Aug 20. |
| DOI: | 10.1094/PHYTO-03-24-0090-R |
| Abstrakt: | Mixtures of fungicides with different modes of action are commonly used as disease and resistance management tools, but little is known of mixtures of natural and synthetic products. In this study, mixtures of metabolites from the rhizobacterium Pseudomonas chlororaphis strain ASF009 formulated as Howler EVO with below-label rates (50 µg/ml) of conventional sterol demethylation inhibitor (DMI) fungicides were investigated for control of anthracnose of cherry ( Prunus avium ) caused by Colletotrichum siamense . Howler mixed with metconazole or propiconazole synergistically reduced disease severity through lesion growth. Real-time PCR showed that difenoconazole, flutriafol, metconazole, and propiconazole induced the expression of DMI target genes CsCYP51A and CsCYP51B in C. siamense . The addition of Howler completely suppressed the DMI fungicide-induced expression of both CYP51 genes. We hypothesize that the downregulation of DMI fungicide-induced expression of the DMI target genes may, at least in part, explain the synergism observed in detached fruit assays. Competing Interests: The author(s) declare no conflict of interest. |
| Databáze: | MEDLINE |
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