Comparative study on muscle function in two different streptozotocin-induced diabetic models.

Autor: Aisyah R; Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan., Kamesawa M; Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan., Horii M; Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan., Watanabe D; Graduate School of Humanities and Social Sciences, Hiroshima University, Hiroshima, 739-8521, Japan.; Graduate School of Sport and Health Sciences, Osaka University of Health and Sport Sciences, Osaka, 564-8565, Japan., Yoshida Y; Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan., Miyata K; Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan., Kumrungsee T; Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan., Wada M; Graduate School of Humanities and Social Sciences, Hiroshima University, Hiroshima, 739-8521, Japan., Yanaka N; Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan. yanaka@hiroshima-u.ac.jp.
Jazyk: angličtina
Zdroj: Acta diabetologica [Acta Diabetol] 2024 Nov; Vol. 61 (11), pp. 1443-1453. Date of Electronic Publication: 2024 Jun 10.
DOI: 10.1007/s00592-024-02311-3
Abstrakt: Aims: Streptozotocin (STZ) is widely used to study diabetic complications. Owing to the nonspecific cytotoxicity of high-dose STZ, alternative models using moderate-dose or a combination of low-dose STZ and a high-fat diet have been established. This study aimed to investigate the effects of these models on muscle function.
Methods: The muscle function of two STZ models using moderate-dose STZ (100 mg/kg, twice) and a combination of low-dose STZ and high-fat diet (50 mg/kg for 5 consecutive days + 45% high-fat diet) were examined using in vivo electrical stimulation. Biochemical and gene expression analysis were conducted on the skeletal muscles of the models immediately after the stimulation.
Results: The contractile force did not differ significantly between the models compared to respective controls. However, the moderate-dose STZ model showed more severe fatigue and blunted exercise-induced glycogen degradation possibly thorough a downregulation of oxidative phosphorylation- and vasculature development-related genes expression.
Conclusions: Moderate-dose STZ model is suitable for fatigability assessment in diabetes and careful understanding on the molecular signatures of each model is necessary to guide the selection of suitable models to study diabetic myopathy.
(© 2024. The Author(s).)
Databáze: MEDLINE
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