The pioneer transcription factor Zelda facilitates the exit from regeneration and restoration of patterning in Drosophila .

Autor:	Bose A; Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA., Schuster K; Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA., Kodali C; Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA., Sonam S; Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA., Smith-Bolton R; Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 May 30. Date of Electronic Publication: 2024 May 30.
DOI:	10.1101/2024.05.30.596672
Abstrakt:	For a damaged tissue to regenerate, the injured site must repair the wound, proliferate, and restore the correct patterning and cell types. We found that Zelda, a pioneer transcription factor largely known for its role in embryonic zygotic genome activation, is dispensable for normal wing development but crucial for wing disc patterning during regeneration. Impairing Zelda function during disc regeneration resulted in adult wings with a plethora of cell fate errors, affecting the veins, margins, and posterior compartment identity. Using CUT&RUN, we identified and validated targets of Zelda including the cell fate genes cut, Delta and achaete , which failed to return to their normal expression patterns upon loss of Zelda. In addition, Zelda controls expression of factors previously established to preserve cell fate during regeneration like taranis and osa, which stabilizes engrailed expression during regeneration, thereby preserving posterior identity. Finally, Zelda ensures proper expression of the integrins encoded by multiple edematous wings and myospheroid during regeneration to prevent blisters in the resuting adult wing. Thus, Zelda is crucial for maintaining cell fate and structural architecture of the regenerating tissue. Competing Interests: Declaration of interests The authors declare no competing interests. K.S.’s current institutional affiliation is the Center for Developmental Genetics, New York University. S.S.’s current institutional affiliation is the Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign.
Databáze:	MEDLINE
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