Osteocalcin improves testicular morphology but does not ameliorate testosterone synthesis signaling in azoospermic mice.

Autor:	Yaghobinejad M; Anatomy Department, Medical School, Tehran University of Medical Science, Tehran, Iran., Toolee H; School of Allied Medical Sciences, Shahrud University of Medical Sciences, Shahrud, Iran., Solhjoo S; Anatomy Department, Medical School, Kerman University of Medical Science, Tehran, Iran., Seifali E; Anatomy Department, Azad Islamic University of Medical Science, Tehran, Iran., Parvari S; Anatomy Department, School of Medicine, Alborz University of Medical Science, Karaj, Iran., Akanji OD; Anatomy Department, Olabisi Onabanjo University, Sagamu, Nigeria., Rastegar T; Anatomy Department, Medical School, Tehran University of Medical Science, Tehran, Iran.
Jazyk:	angličtina
Zdroj:	Clinical and experimental reproductive medicine [Clin Exp Reprod Med] 2024 Dec; Vol. 51 (4), pp. 344-352. Date of Electronic Publication: 2024 Jun 10.
DOI:	10.5653/cerm.2023.06674
Abstrakt:	Objective: Osteocalcin (OCN) influences spermatogenesis in conjunction with testosterone and estrogen. OCN facilitates the secretion of testosterone by engaging with G protein-coupled receptor class C group 6 member A (GPRC6A) on Leydig cells and with androgen receptors on Sertoli cells. Methods: Adult mice were assigned to the following groups: control; sham I, which received dimethyl sulfoxide for 5 weeks followed by phosphate-buffered saline for 1 month; azoospermia, which was treated with busulfan (40 mg/kg); sham II, which consisted of azoospermic animals that received phosphate-buffered saline for 1 month beginning at the 5-week mark; and the experimental group, which included azoospermic mice treated with OCN (3 ng/g/day) for 1 month. Results: In the mice receiving OCN treatment, immunohistochemical analysis revealed increased expression of androgen receptors and GPRC6A, indicative of enhanced spermatogenesis. Additionally, the expression levels of the cyclic adenosine monophosphate-responsive element binding protein 1, steroidogenic acute regulatory protein, and cytochrome P450 family 11 genes were elevated. However, testosterone levels exhibited no significant differences across groups. Morphometric analysis suggests that OCN may play a crucial role in spermatogenesis, as evidenced by its positive effects on germinal cells and the germinal epithelium in the azoospermia group (p<0.05). Conclusion: We conclude that OCN may serve as a beneficial therapeutic agent for male infertility.
Databáze:	MEDLINE
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