Protective effects of the secondary metabolites from Quercus salicina Blume against gentamicin-induced nephrotoxicity in zebrafish (Danio rerio) model.

Autor: Yin SH; School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun, 130117, Jilin Province, China., Zhang WJ; School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun, 130117, Jilin Province, China., Jiang LL; School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun, 130117, Jilin Province, China., Wang GY; School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun, 130117, Jilin Province, China., Jeon YJ; School of Marine Biomedical Science, Je Ju National University, Je ju Daehakro, Je ju City 63243, Republic of Korea., Ding Y; School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun, 130117, Jilin Province, China., Li Y; School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun, 130117, Jilin Province, China. Electronic address: liyong@ccucm.edu.cn.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2024 Sep; Vol. 283, pp. 109952. Date of Electronic Publication: 2024 Jun 08.
DOI: 10.1016/j.cbpc.2024.109952
Abstrakt: To reveal the protective effect on the nephrotoxicity of Quercus salicina Blume(QS), a traditional medicine for the treatment of urolithiasis, the 50 % ethanol extract from the branches and leaves of QS was chemically studied by systematic solvent extraction and HPLC chromatography. Two phenolic acids and three flavonoids were identified by nuclear magnetic resonance spectroscopy, namely Ferulic acid (1), p-Hydroxycinnamic acid (2), Hesperidin (3), Formononetin (4), and Quercetin (5). At the same time, the gentamicin-induced nephrotoxicity of zebrafish was used as a model for the first time. The antioxidant activity of these derivatives with good antioxidant activity screened from free radical scavenging experiments in vitro (DPPH and ABTS) was evaluated in vivo, including protein levels (LPO, NO, GSH, and SOD), kidney injury factor (KIM-1), zebrafish kidney pathology and real-time PCR. The results showed that metabolites 1, 3, and 5 had strong antioxidant activity, and oxidative stress in renal tissue was significantly reduced; KIM-1, TNF-α, and IL-6 mRNA expression in a dose-dependent manner, which preliminarily revealed the protective effect of the secondary metabolites of QS on nephrotoxicity, and preliminarily discussed the structure-activity relationship. This study provides an experimental basis for further exploring the mechanism of QS in the kidney.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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