Enhancement of triclocarban biodegradation: Metabolic division of labor in co-culture of Rhodococcus sp. BX2 and Pseudomonas sp. LY-1.

Autor: Hong Y; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China., Sun G; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China., Sun S; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China; Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, Department of Bioengineering, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin, 150080, PR China., Miao L; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China., Yang H; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China., Wu B; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China., Ma T; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China., Chen S; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China., Sun L; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China., Yang J; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China., Sun Y; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China., Liu Y; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China., Zang H; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China; Key Laboratory of Swine Facilities Engineering, Ministry of Agriculture and Rural Affairs, Harbin, 150030, PR China., Li C; College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China; Key Laboratory of Swine Facilities Engineering, Ministry of Agriculture and Rural Affairs, Harbin, 150030, PR China. Electronic address: chunyanli@neau.edu.cn.
Jazyk: angličtina
Zdroj: Environmental pollution (Barking, Essex : 1987) [Environ Pollut] 2024 Sep 01; Vol. 356, pp. 124346. Date of Electronic Publication: 2024 Jun 07.
DOI: 10.1016/j.envpol.2024.124346
Abstrakt: Triclocarban (TCC) and its metabolite, 3,4-dichloroaniline (DCA), are classified as emerging organic contaminants (EOCs). Significant concerns arise from water and soil contamination with TCC and its metabolites. These concerns are especially pronounced at high concentrations of up to approximately 20 mg/kg dry weight, as observed in wastewater treatment plants (WWTPs). Here, a TCC-degrading co-culture system comprising Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 was utilized to degrade TCC (14.5 mg/L) by 85.9% in 7 days, showing improved degradation efficiency compared with monocultures. A combination of high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), genome sequencing, transcriptomic analysis, and quantitative reverse transcription-PCR (qRT-PCR) was performed. Meanwhile, through the combination of further experiments involving heterologous expression and gene knockout, we proposed three TCC metabolic pathways and identified four key genes (tccG, tccS, phB, phL) involved in the TCC degradation process. Moreover, we revealed the internal labor division patterns and connections in the co-culture system, indicating that TCC hydrolysis products were exchanged between co-cultured strains. Additionally, mutualistic cooperation between BX2 and LY-1 enhances TCC degradation efficiency. Finally, phytotoxicity assays confirmed a significant reduction in the plant toxicity of TCC following synergistic degradation by two strains. The in-depth understanding of the TCC biotransformation mechanisms and microbial interactions provides useful information for elucidating the mechanism of the collaborative biodegradation of various contaminants.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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