The mechanistic insights of essential oil of Mentha piperita to control Botrytis cinerea and the prospection of lipid nanoparticles to its application.

Autor: Fuentes JM; Centro de Excelencia en Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA), Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Av. Francisco Salazar, P.O. Box 54-D, Temuco 01145, Chile., Jofré I; Scientific and Technological Bioresource Nucleus (BIOREN-UFRO), Universidad de La Frontera, Av. Francisco Salazar, Casilla 54-D, Temuco 01145, Chile., Tortella G; Centro de Excelencia en Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA), Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Av. Francisco Salazar, P.O. Box 54-D, Temuco 01145, Chile; Department of Chemical Engineering. Faculty of Engineering and Sciences, Universidad de La Frontera, Av. Francisco Salazar, Casilla 54-D, Temuco 01145, Chile., Benavides-Mendoza A; Horticulture Department, Agronomy Division, Universidad Autónoma Agraria Antonio Narro, Saltillo, Mexico., Diez MC; Centro de Excelencia en Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA), Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Av. Francisco Salazar, P.O. Box 54-D, Temuco 01145, Chile; Department of Chemical Engineering. Faculty of Engineering and Sciences, Universidad de La Frontera, Av. Francisco Salazar, Casilla 54-D, Temuco 01145, Chile., Rubilar O; Centro de Excelencia en Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA), Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Av. Francisco Salazar, P.O. Box 54-D, Temuco 01145, Chile; Department of Chemical Engineering. Faculty of Engineering and Sciences, Universidad de La Frontera, Av. Francisco Salazar, Casilla 54-D, Temuco 01145, Chile., Fincheira P; Centro de Excelencia en Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA), Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Av. Francisco Salazar, P.O. Box 54-D, Temuco 01145, Chile; Department of Chemical Engineering. Faculty of Engineering and Sciences, Universidad de La Frontera, Av. Francisco Salazar, Casilla 54-D, Temuco 01145, Chile. Electronic address: p.fincheira01@ufromail.cl.
Jazyk: angličtina
Zdroj: Microbiological research [Microbiol Res] 2024 Sep; Vol. 286, pp. 127792. Date of Electronic Publication: 2024 May 29.
DOI: 10.1016/j.micres.2024.127792
Abstrakt: Botrytis cinerea is the phytopathogenic fungus responsible for the gray mold disease that affects crops worldwide. Essential oils (EOs) have emerged as a sustainable tool to reduce the adverse impact of synthetic fungicides. Nevertheless, the scarce information about the physiological mechanism action and the limitations to applying EOs has restricted its use. This study focused on elucidating the physiological action mechanisms and prospection of lipid nanoparticles to apply EO of Mentha piperita. The results showed that the EO of M. piperita at 500, 700, and 900 μL L -1 inhibited the mycelial growth at 100 %. The inhibition of spore germination of B. cinerea reached 31.43 % at 900 μL L -1 . The EO of M. piperita decreased the dry weight and increased pH, electrical conductivity, and cellular material absorbing OD 260 nm of cultures of B. cinerea. The fluorescence technique revealed that EO reduced hyphae width, mitochondrial activity, and viability, and increased ROS production. The formulation of EO of M. piperita loaded- solid lipid nanoparticles (SLN) at 500, 700, and 900 μL L -1 had particle size ∼ 200 nm, polydispersity index < 0.2, and stability. Also, the thermogravimetric analysis indicated that the EO of M. piperita-loaded SLN has great thermal stability at 50 °C. EO of M. piperita-loaded SLN reduced the mycelial growth of B. cinerea by 70 %, while SLN formulation (without EO) reached 42 % inhibition. These results supported that EO of M. piperita-loaded SLN is a sustainable tool for reducing the disease produced by B. cinerea.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2024 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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