Bioprospecting endophytic fungi for bioactive metabolites with seed germination promoting potentials.

Autor: El-Nagar D; Soil and Water Research Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Cairo, Egypt., Salem SH; Department of Microbiology, Faculty of Agriculture, Zagazig University, Zagazig, Egypt., El-Zamik FI; Department of Microbiology, Faculty of Agriculture, Zagazig University, Zagazig, Egypt., El-Basit HMIA; Department of Microbiology, Faculty of Agriculture, Zagazig University, Zagazig, Egypt., Galal YGM; Soil and Water Research Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Cairo, Egypt., Soliman SM; Soil and Water Research Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Cairo, Egypt., Aziz HAA; Soil and Water Research Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Cairo, Egypt., Rizk MA; Soil and Water Research Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Cairo, Egypt., El-Sayed ER; Plant Research Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Cairo, Egypt. sayed_zahran2000@yahoo.com.
Jazyk: angličtina
Zdroj: BMC microbiology [BMC Microbiol] 2024 Jun 08; Vol. 24 (1), pp. 200. Date of Electronic Publication: 2024 Jun 08.
DOI: 10.1186/s12866-024-03337-x
Abstrakt: There is an urgent need for new bioactive molecules with unique mechanisms of action and chemistry to address the issue of incorrect use of chemical fertilizers and pesticides, which hurts both the environment and the health of humans. In light of this, research was done for this work to isolate, identify, and evaluate the germination-promoting potential of various plant species' fungal endophytes. Zea mays L. (maize) seed germination was examined using spore suspension of 75 different endophytic strains that were identified. Three promising strains were identified through screening to possess the ability mentioned above. These strains Alternaria alternate, Aspergilus flavus, and Aspergillus terreus were isolated from the stem of Tecoma stans, Delonix regia, and Ricinus communis, respectively. The ability of the three endophytic fungal strains to produce siderophore and indole acetic acid (IAA) was also examined. Compared to both Aspergillus flavus as well as Aspergillus terreus, Alternaria alternata recorded the greatest rates of IAA, according to the data that was gathered. On CAS agar versus blue media, all three strains failed to produce siderophores. Moreover, the antioxidant and antifungal potentials of extracts from these fungi were tested against different plant pathogens. The obtained results indicated the antioxidant and antifungal activities of the three fungal strains. GC-Mass studies were carried out to determine the principal components in extracts of all three strains of fungi. The three strains' fungus extracts included both well-known and previously unidentified bioactive compounds. These results may aid in the development of novel plant growth promoters by suggesting three different fungal strains as sources of compounds that may improve seed germination. According to the study that has been given, as unexplored sources of bioactive compounds, fungal endophytes have great potential.
(© 2024. The Author(s).)
Databáze: MEDLINE
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