Effects and mechanisms of sciadonic acid on colonic transit function through regulating 5-HT4/cAMP/PKA/AQP4 signaling pathway in STC model mice.

Autor: Yu Z; School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China., Zikela L; School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China., Wang D; School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China., Wang X; School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China., Zhu H; School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China., Li S; School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China; Academy of Chinese Medical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China., Han Q; School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China; Academy of Chinese Medical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. Electronic address: hanqiang@zcmu.edu.cn.
Jazyk: angličtina
Zdroj: The Journal of nutritional biochemistry [J Nutr Biochem] 2024 Sep; Vol. 131, pp. 109676. Date of Electronic Publication: 2024 Jun 06.
DOI: 10.1016/j.jnutbio.2024.109676
Abstrakt: Torreya grandis (T. grandis) oil has been reported to alleviate symptoms of slow transit constipation (STC). However, the impact of sciadonic acid (SA), a distinctive fatty acid found in T. grandis oil, on the pathological progression of STC remains unclear. This study aimed to evaluate the effect of SA on STC and uncover the underlying mechanisms. The STC model was established by feeding Balb/c mice with loperamide. After 2 weeks of intervention, SA significantly improved weight loss and intestinal motility decline induced by STC, along with enhancing plasma indices and reducing colon pathological damage. SA effectively reversed the STC-induced decrease in the 5-HT4/cAMP/PKA/AQP4 signaling pathway genes and expression. Furthermore, 16S rRNA analysis demonstrated that SA mitigated the imbalance of the intestinal microbiota induced by STC, by reducing the ratio of Firmicutes to Bacteroidetes (F/B) and increasing the abundance of beneficial bacteria such as Akkermansia. In conclusion, SA intervention alleviated colonic dysfunction in STC mice. The activation of the SA-mediated 5-HT4/cAMP/PKA/AQP4 signaling pathway may serve as a potential target for STC treatment. These findings suggest that SA holds promise as a treatment option for STC and could potentially be extended to other related gut diseases for further investigation.
Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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