Participation of kisspeptin, progesterone, and GnRH receptors on lordosis behavior induced by kisspeptin.

Autor: González-Flores O; Centro de Investigación en Reproducción Animal, Universidad Autónoma de Tlaxcala-CINVESTAV, Tlaxcala, México., Domínguez-Ordóñez R; Licenciatura en Ingeniería Agronómica y Zootecnia, CRC, Benemérita Universidad Autónoma de, Puebla, México., Delgado-Macuil RJ; Centro de Investigación en Biotecnología Aplicada, Instituto Politécnico Nacional, Santa Inés, Tecuexcomac, Tlaxcala, México., Tlachi-López JL; Instituto de Investigaciones Cerebrales. Universidad Veracruzana, Veracruz, México., Luna-Hernández A; Doctorado en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, México., Montes-Narváez O; Doctorado en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, México., Pfaus JG; Center for Sexual Health and Intervention, Czech National Institute of Mental Health, Klecany, Czech Republic; Department of Psychology and Life Sciences, Charles University, Prague, Czech Republic., García-Juárez M; Centro de Investigación en Reproducción Animal, Universidad Autónoma de Tlaxcala-CINVESTAV, Tlaxcala, México. Electronic address: mgarciaj@uatx.mx.
Jazyk: angličtina
Zdroj: Physiology & behavior [Physiol Behav] 2024 Sep 01; Vol. 283, pp. 114609. Date of Electronic Publication: 2024 Jun 06.
DOI: 10.1016/j.physbeh.2024.114609
Abstrakt: The neuropeptide kisspeptin (Kiss) is crucial in regulating the hypothalamic-pituitary-gonadal axis. It is produced by two main groups of neurons in the hypothalamus: the rostral periventricular region around the third ventricle and the arcuate nucleus. Kiss is the peptide product of the KiSS-1 gene and serves as the endogenous agonist for the GPR54 receptor. The Kiss/GPR54 system functions as a critical regulator of the reproductive system. Thus, we examined the effect of intracerebroventricular administration of 3 μg of Kiss to the right lateral ventricle of ovariectomized rats primed with a dose of 5 μg subcutaneous (sc) of estradiol benzoate (EB). Kiss treatment increased the lordosis quotient at all times tested. However, the lordosis reflex score was comparatively lower yet still significant compared to the control group. To investigate receptor specificity and downstream mechanisms on lordosis, we infused 10 μg of GPR54 receptor antagonist, Kiss-234, 5 μg of the progestin receptor antagonist, RU486, or 3 μg of antide, a gonadotropin-releasing hormone-1 (GnRH-1) receptor antagonist, to the right lateral ventricle 30 min before an infusion of 3 μg of Kiss. Results demonstrated a significant reduction in the facilitation of lordosis behavior by Kiss at 60 and 120 min when Kiss-234, RU486, or antide were administered. These findings suggest that Kiss stimulates lordosis expression by activating GPR54 receptors on GnRH neurons and that Kiss/GPR54 system is an essential intermediary by which progesterone activates GnRH.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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