Insights on kraft lignin degradation in an anaerobic environment.

Autor: Silva JP; Enzymology Laboratory, Cell Biology Department, Universidade de Brasília (UnB), Brasília 70910-900, Brazil., Frederico TD; Enzymology Laboratory, Cell Biology Department, Universidade de Brasília (UnB), Brasília 70910-900, Brazil., Ticona ARP; Enzyme Biotechnology Research Laboratory, Science Faculty, Universidad Nacional Jorge Basadre Grohmann, Tacna 23003, Peru., Pinto OHB; Genomic for Climate Change Research Center (GCCRC), Universidade Estadual de Campinas (UNICAMP), Campinas, SP 13083-875, Brazil., Williams TCR; Plant Biochemistry Laboratory, Department of Botany, University of Brasilia, Brasília 70910-900, Brazil., Krüger RH; Enzymology Laboratory, Cell Biology Department, Universidade de Brasília (UnB), Brasília 70910-900, Brazil., Noronha EF; Enzymology Laboratory, Cell Biology Department, Universidade de Brasília (UnB), Brasília 70910-900, Brazil. Electronic address: enoronha@unb.br.
Jazyk: angličtina
Zdroj: Enzyme and microbial technology [Enzyme Microb Technol] 2024 Sep; Vol. 179, pp. 110468. Date of Electronic Publication: 2024 Jun 03.
DOI: 10.1016/j.enzmictec.2024.110468
Abstrakt: Lignin is an aromatic macromolecule and one of the main constituents of lignocellulosic materials. Kraft lignin is generated as a residual by-product of the lignocellulosic biomass industrial process, and it might be used as a feedstock to generate low molecular weight aromatic compounds. In this study, we seek to understand and explore the potential of ruminal bacteria in the degradation of kraft lignin. We established two consortia, KLY and KL, which demonstrated significant lignin-degrading capabilities. Both consortia reached maximum growth after two days, with KLY showing a higher growth and decolorization rate. Additionally, SEM analysis revealed morphological changes in the residual lignin from both consortia, indicating significant degradation. This was further supported by FTIR spectra, which showed new bands corresponding to the C-H vibrations of guaiacyl and syringyl units, suggesting structural transformations of the lignin. Taxonomic analysis showed enrichment of the microbial community with members of the Dickeya genus. Seven metabolic pathways related to lignin metabolism were predicted for the established consortia. Both consortia were capable of consuming aromatic compounds such as 4-hydroxybenzoic acid, syringaldehyde, acetovanillone, and syringic acid, highlighting their capacity to convert aromatic compounds into commercially valuable molecules presenting antifungal activity and used as food preservatives as 4-hydroxyphenylacetic, 3-phenylacetic, and phenylacetic acids. Therefore, the microbial consortia shown in the present work are models for understanding the process of lignin degradation and consumption in bacterial anaerobic communities and developing biological processes to add value to industrial processes based on lignocellulosic biomass as feedstock.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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