Alkalimonas mucilaginosa sp. nov. and Alkalimonas cellulosilytica sp. nov. isolated from alkaline Lonar lake, India.

Autor: Thite S; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, NCCS Complex, Savitribai Phule Pune University Campus, Ganeshkhind Road, Pune, 411007, Maharashtra State, India., Godbole D; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, NCCS Complex, Savitribai Phule Pune University Campus, Ganeshkhind Road, Pune, 411007, Maharashtra State, India., Debnath M; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, NCCS Complex, Savitribai Phule Pune University Campus, Ganeshkhind Road, Pune, 411007, Maharashtra State, India., Bhatt A; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, NCCS Complex, Savitribai Phule Pune University Campus, Ganeshkhind Road, Pune, 411007, Maharashtra State, India., Yadav A; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, NCCS Complex, Savitribai Phule Pune University Campus, Ganeshkhind Road, Pune, 411007, Maharashtra State, India., Lodha T; Bioenergy Group, Agharkar Research Institute, Gopal Ganesh Agarkar Road, Pune, 411004, Maharashtra, India., Joseph N; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, NCCS Complex, Savitribai Phule Pune University Campus, Ganeshkhind Road, Pune, 411007, Maharashtra State, India., Kirdat K; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, NCCS Complex, Savitribai Phule Pune University Campus, Ganeshkhind Road, Pune, 411007, Maharashtra State, India., Boruah D; Department of Pathology, Armed Forces Medical College, Pune, 411040, India., Sharma R; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.; Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Chandigarh, 160036, India., Joshi A; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, NCCS Complex, Savitribai Phule Pune University Campus, Ganeshkhind Road, Pune, 411007, Maharashtra State, India. amaraja@nccs.res.in.
Jazyk: angličtina
Zdroj: Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2024 Jun 08; Vol. 117 (1), pp. 88. Date of Electronic Publication: 2024 Jun 08.
DOI: 10.1007/s10482-024-01986-6
Abstrakt: Two alkaliphilic, Gram-stain-negative bacterial strains (MEB004 T and MEB108 T ) were isolated from water samples collected from Lonar lake, India. The phylogenetic analysis of their 16S rRNA gene sequences showed the highest similarity to A. delamerensis DSM 18314 T (98.4%), followed by A. amylolytica DSM 18337 T and A. collagenimarina JCM 14267 T (97.9%). The genome sizes of strains MEB004 T and MEB108 T were determined to be 3,858,702 and 4,029,814 bp, respectively, with genomic DNA G + C contents of 51.4 and 51.9%. Average Nucleotide Identity, DNA-DNA Hybridization and Amino Acid Identity values between strains (MEB004 T and MEB108 T ) and A. amylolytica DSM 18337 T were (82.3 and 85.5), (25.0 and 29.2) and (86.7 and 90.2%). Both novel strains produced industrially important enzymes, such as amylase, lipase, cellulase, caseinase, and chitinase at pH 10 evidenced by the genomic presence of carbohydrate-active enzymes encoding genes. Genomic analyses further identified pH tolerance genes, affirming their adaptation to alkaline Lonar Lake. Dominant fatty acids were Summed feature 8 (C 18:1 ω7c and/or C 18:1 ω6c), C 16:0, Summed feature 3, Sum In Feature 2 and C 12:0 3OH. The prevalent polar lipids included phosphatidyl ethanolamine, phosphatidyl glycerol, and diphosphatidyl glycerol. The major respiratory quinone was ubiquinone-8. Based on the polyphasic data, we propose the classification of strains MEB004 T and MEB108 T as novel species within the genus Alkalimonas assigning the names Alkalimonas mucilaginosa sp. nov. and Alkalimonas cellulosilytica sp. nov., respectively. The type strains are MEB004 T (= MCC 5208 T  = JCM 35954 T  = NCIMB 15460 T ) and MEB108 T (= MCC 5330 T  = JCM 35955 T  = NCIMB 15461 T ).
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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